Cholate
Mostrando 13-24 de 130 artigos, teses e dissertações.
-
13. Cholate-dependent killing of Giardia lamblia by human milk.
We showed previously that nonimmune human milk (NHM) kills Giardia lamblia trophozoites in vitro and presented evidence that killing requires the bile salt-stimulated lipase of milk. Since this enzyme is activated by bile salts, killing should be dependent on the presence of bile salts. We now show that killing by fresh NHM or NHM stored at -70 degrees C is
-
14. Effect of water-soluble polymers on the state of aggregation, vesicle size, and phase transformations in mixtures of phosphatidylcholine and sodium cholate.
The state of aggregation and the steady-state size of mixed aggregates made of phospholipids and surfactants are both determined by the surfactant/lipid ratio in the mixed aggregates (Re). Water-soluble polymers, such as dextrans and polyethylene glycols (PEGs) of different molecular weights, induce reversible aggregation of phospholipid vesicles, mostly due
-
15. Pancreatic carboxyl ester lipase: a circulating enzyme that modifies normal and oxidized lipoproteins in vitro.
Pancreatic carboxyl ester lipase (CEL) hydrolyzes cholesteryl esters (CE), triglycerides (TG), and lysophospholipids, with CE and TG hydrolysis stimulated by cholate. Originally thought to be confined to the gastrointestinal system, CEL has been reported in the plasma of humans and other mammals, implying its potential in vivo to modify lipids associated wit
-
16. Efficiency of various bile salt preparations for stimulation of Clostridium difficile spore germination.
Taurocholate, desoxycholate, and cholate stimulated germination of Clostridium difficile spores in broth medium and enhanced recovery of C. difficile spores on a selective agar medium. Desoxycholate and some crude taurocholate preparations also inhibited multiplication of vegetative cells. At a concentration of 1.2 X 10(-2) M, sodium cholate inhibited multip
-
17. Preferential inhibitory action of sodium cholate on an Escherichia coli strain carrying a plasmid in an integrated state.
Sodium cholate was shown to be preferentially more active on Escherichia coli strains carrying an integrated plasmid, i.e., on Hfr strains, than on their parental strains with or without a plasmid in an autonomous state.
-
18. Cholic acid mediates negative feedback regulation of bile acid synthesis in mice
Cholesterol is converted into dozens of primary and secondary bile acids through pathways subject to negative feedback regulation mediated by the nuclear receptor farnesoid X receptor (FXR) and other effectors. Disruption of the sterol 12α-hydroxylase gene (Cyp8b1) in mice prevents the synthesis of cholate, a primary bile acid, and its metabolites. Feedback
American Society for Clinical Investigation.
-
19. Studies on the Reconstitution of O2-Evolution of Chloroplasts 1
Extraction of spinach (Spinacia oleracea L.) chloroplasts with cholate-asolectin in the absence of Mg2+ results in the rapid and selective inactivation of O2 evolution and a partial (30 to 40%) loss of photosystem II (PSII) donor activity without extraction of thylakoid bound Mn (∼5 to 6 Mn per 400 Chlorophyll). Inclusion of ethylene glycol in the extracti
-
20. Effects of Nonpolar Mutations in Each of the Seven Bacillus subtilis mrp Genes Suggest Complex Interactions among the Gene Products in Support of Na+ and Alkali but Not Cholate Resistance
The Bacillus subtilis mrp (multiple resistance and pH) operon supports Na+ and alkali resistance via an Na+/H+ antiport, as well as cholate efflux and resistance. Among the individual mutants with nonpolar mutations in each of the seven mrp genes, only the mrpF mutant exhibited cholate sensitivity and a cholate efflux defect that were complemented by express
American Society for Microbiology.
-
21. Characterization of the baiH gene encoding a bile acid-inducible NADH:flavin oxidoreductase from Eubacterium sp. strain VPI 12708.
A cholate-inducible, NADH-dependent flavin oxidoreductase from the intestinal bacterium Eubacterium sp. strain VPI 12708 was purified 372-fold to apparent electrophoretic homogeneity. The subunit and native molecular weights were estimated to be 72,000 and 210,000, respectively, suggesting a homotrimeric organization. Three peaks of NADH:flavin oxidoreductas
-
22. Expression in Xenopus oocytes of rat liver mRNA coding for a bile salt-dependent cholesteryl ester hydrolase.
A catalytically active bile salt-dependent cholesteryl ester hydrolase (CEH) was expressed when Xenopus oocytes were injected with rat liver mRNA. The expressed CEH activity was highly dependent on the presence of trihydroxy bile salts (cholate or one of its conjugates); maximum hydrolytic activity was observed in the presence of 10 mM sodium cholate. The ex
-
23. Immunological detection of the Kanagawa phenomenon of vibrio parahaemolyticus on modified selective media.
Selective media for Vibrio parahaemolyticus, BTB-Teepol agar and modified arabinose-ammonium sulfate-cholate agar, were modified for use in immunological detection of the thermostable direct hemolysin produced by this organism. The modified BTB-Teepol agar and modified arabinose-ammonium sulfate-cholate agar were both found to be useful for the modified Elek
-
24. OBSERVATIONS CONCERNING THE CHOLATE: CHOLESTEROL RELATIONSHIP IN CLINICAL AND EXPERIMENTAL NEPHROSIS 12