Cells Immobilization
Mostrando 1-12 de 153 artigos, teses e dissertações.
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1. Advances in enzyme bioelectrochemistry
ABSTRACT Bioelectrochemistry can be defined as a branch of Chemical Science concerned with electron-proton transfer and transport involving biomolecules, as well as electrode reactions of redox enzymes. The bioelectrochemical reactions and system have direct impact in biotechnological development, in medical devices designing, in the behavior of DNA-protein
An. Acad. Bras. Ciênc.. Publicado em: 2018
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2. Statistical Modelling and Optimization of Fermentation Medium for Lincomycin Production by Streptomyces lincolnensis Immobilized Cells
ABSTRACT Response surface methodology was used to optimize lincomycin production by Streptomyces lincolnensis NRRL ISP-5355 in submerged fermentation. Screening of fermentation medium components to find their relative effect on lincomycin production was done using Plackett-Burman design. Malt extract, dextrin, soluble starch and (NH4)2SO4 were the most signi
Braz. arch. biol. technol.. Publicado em: 11/05/2017
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3. Zymomonas mobilis IMMOBILIZED ON LOOFA SPONGE AND SUGARCANE BAGASSE FOR LEVAN AND ETHANOL PRODUCTION USING REPEATED BATCH FERMENTATION
Abstract Two experiments were carried out using loofa sponge and sugarcane bagasse as immobilization support. First a 2(5-2) design of experiments was realized with the following independent variables: sucrose concentration; pH; incubation time and agitation. Based on the best results observed in this experiment, the capacity for reuse of the supports in sub
Braz. J. Chem. Eng.. Publicado em: 2017-04
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4. SYNTHESIS, CHARACTERIZATION AND APPLICATION OF A POLYURETHANE-BASED SUPPORT FOR IMMOBILIZING MEMBRANE-BOUND LIPASE
Abstract This study conducted an assessment of polyurethane foams that were synthesized by one-shot process and used as a low-cost support to immobilize Mucor circinelloides URM 4182 whole-cells presenting high lipolytic activity. Polyols with different molecular weights (1100 to 6000 g mol-1) were applied to synthesize the polymer matrix, and the agitation
Braz. J. Chem. Eng.. Publicado em: 2017-01
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5. Three days of intermittent stretching after muscle disuse alters the proteins involved in force transmission in muscle fibers in weanling rats
The aim of this study was to determine the effects of intermittent passive manual stretching on various proteins involved in force transmission in skeletal muscle. Female Wistar weanling rats were randomly assigned to 5 groups: 2 control groups containing 21- and 30-day-old rats that received neither immobilization nor stretching, and 3 test groups that rece
Braz J Med Biol Res. Publicado em: 04/12/2015
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6. Effects of aluminum on the elongation and external morphology of root tips in two maize genotypes
ABSTRACT The objective of this study was to evaluate the effects of toxic levels of aluminum (Al) on the growth and external morphology of root tips in two maize genotypes with differential Al tolerance. The maize genotypes UFVM-100 (Al-sensitive) and UFVM-200 (Al-tolerant) were treated with 0 and 50 mM Al in a 0.5 mM CaCl2 solution at pH 4.5; root elongatio
Bragantia. Publicado em: 27/11/2015
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7. PERFORMANCE OF LOOFA-IMMOBILIZED Rhizopus oryzae IN THE ENZYMATIC PRODUCTION OF BIODIESEL WITH USE OF OLEIC ACID IN n-HEXANE MEDIUM
AbstractThe performance of loofa-immobilized Rhizopus oryzae (as a whole-cell biocatalyst) in the synthesis of methyl oleate was evaluated using oleic acid as a model substrate. The activities of the cell-bound lipase in terms of the esterification and hydrolysis reactions were found to be higher for the immobilized cells as compared with those of the free c
Braz. J. Chem. Eng.. Publicado em: 2015-06
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8. Surface characterization and osteoblast-like Cells culture on collagen modified PLDLA scaffolds
Surface modification techniques based on the grafting of chemical functional groups and immobilization of bioactive molecules have been used to improve biocompatibility and clinical performance of bioabsorbable scaffolds in tissue engineering and medicine regenerative applications. This study aimed at developing and characterizing a biomimetic surface to sti
Mat. Res.. Publicado em: 2014-12
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9. Whole cells in enantioselective reduction of benzyl acetoacetate
The β-ketoester benzyl acetoacetate was enantioselectively reduced to benzyl (S)-3-hydroxybutanoate by seven microorganism species. The best result using free cells was obtained with the yeast Hansenula sp., which furnished 97% ee and 85% of conversion within 24 h. After immobilization in calcium alginate spheres, K.marxianus showed to be more stable after
Braz. J. Microbiol.. Publicado em: 2014-09
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10. Isolation and characterization of novel thermophilic lipase-secreting bacteria
The purpose of the present study was to screen and identify the lipase-producing microorganisms from various regions of Iran. Samples collected from hot spring, Persian Gulf, desert area and oil-contaminated soil, were analyzed for thermophilic extracellular-lipase producing organisms. Six strains with high activity on rhodamine B plates were selected for ch
Braz. J. Microbiol.. Publicado em: 2013-12
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11. Enhancement of chitosanase production by cell immobilization of Gongronella sp. JG
Chitosanase production of Gongronella sp. JG cells immobilized in calcium alginate gel and polyurethane foam was compared with that of the free cells, there was a 60% increase in the enzyme yield (2429 U/L) compared to the highest yield obtained from free cells (1513 U/L). The optimal immobilization parameters (concentrations of sodium alginate, calcium chlo
Braz. J. Microbiol.. Publicado em: 09/04/2013
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12. Ethanol fermentation of sugarcane molasses by Zymomonas mobilis MTCC 92 immobilized in Luffa cylindrica L. sponge discs and Ca-alginate matrices
Bio-ethanol production from cane molasses (diluted to 15 % sugar w/v) was studied using the bacterium, Zymomonas mobilis MTCC 92 entrapped in luffa (Luffa cylindrica L.) sponge discs and Ca-alginate gel beads as the immobilizing matrices. At the end of 96 h fermentation, the final ethanol concentrations were 58.7 ± 0.09 and 59.1 ± 0.08 g/l molasses with lu
Braz. J. Microbiol.. Publicado em: 2012-12