Cell Sorting
Mostrando 1-12 de 678 artigos, teses e dissertações.
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1. Increased peripheral blood TCD4+ counts and serum SP-D levels in patients with chronic paracoccidioidomycosis, during and after antifungal therapy
BACKGROUND The main clinical forms of paracoccidioidomycosis (PCM) are the acute/subacute form (AF) and the chronic form (CF), and they both display considerable clinical variability. The immune responses of PCM patients, during and after treatment, remain neglected, mainly in the case of CF patients, due to the high prevalence of pulmonary sequelae. OBJEC
Mem. Inst. Oswaldo Cruz. Publicado em: 2017-11
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2. Comparison of microRNA expression in high-count monoclonal B-cell lymphocytosis and Binet A chronic lymphocytic leukemia
Abstract Background Evidence suggests that monoclonal B-cell lymphocytosis precedes all chronic lymphocytic leukemia cases, although the molecular mechanisms responsible for disease progression are not understood. Aberrant miRNA expression may contribute to the pathogenesis of chronic lymphocytic leukemia. The objective of this study was to compare miRNA ex
Rev. Bras. Hematol. Hemoter.. Publicado em: 2017-09
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3. Telomere length analysis in monoclonal B-cell lymphocytosis and chronic lymphocytic leukemia Binet A
Monoclonal B-cell lymphocytosis (MBL) is an asymptomatic clinical entity characterized by the proliferation of monoclonal B cells not meeting the diagnosis criteria for chronic lymphocytic leukemia (CLL). MBL may precede the development of CLL, but the molecular mechanisms responsible for disease progression and evolution are not completely known. Telomeres
Braz J Med Biol Res. Publicado em: 13/04/2017
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4. Validation of interphase fluorescence in situ hybridization (iFISH) for multiple myeloma using CD138 positive cells
ABSTRACT BACKGROUND: Multiple myeloma is a plasma cell neoplasm with acquired genetic abnormalities of clinical and prognostic importance. Multiple myeloma differs from other hematologic malignancies due to a high fraction of low proliferating malignant plasma cells and the paucity of plasma cells in bone marrow aspiration samples, making cytogenetic analy
Rev. Bras. Hematol. Hemoter.. Publicado em: 2016-06
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5. Yeast CUP1 protects HeLa cells against copper-induced stress
As an essential trace element, copper can be toxic in mammalian cells when present in excess. Metallothioneins (MTs) are small, cysteine-rich proteins that avidly bind copper and thus play an important role in detoxification. YeastCUP1 is a member of the MT gene family. The aim of this study was to determine whether yeast CUP1 could bind copper effectively a
Braz J Med Biol Res. Publicado em: 12/06/2015
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6. Use of the heteroduplex mobility assay and cell sorting to select genome sequences of the CCR5 gene in HEK 293T cells edited by transcription activator-like effector nucleases
Engineered nucleases such as zinc finger nucleases (ZFN) and transcription activator-like effector nucleases (TALEN) are one of the most promising tools for modifying genomes. These site-specific enzymes cause double- strand breaks that allow gene disruption or gene insertion, thereby facilitating genetic manipulation. The major problem associated with this
Genet. Mol. Biol.. Publicado em: 2014
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7. Differential in vitro activity of the DNA topoisomerase inhibitor idarubicin against Trypanosoma rangeli and Trypanosoma cruzi
In this study the effect of eight DNA topoisomerase inhibitors on the growth Trypanosoma rangeli epimastigotes in cell culture was investigated. Among the eight compounds tested, idarubicin was the only compound that displayed promising trypanocidal activity with a half-maximal growth inhibition (GI50) value in the sub-micromolar range. Fluorescence-activate
Mem. Inst. Oswaldo Cruz. Publicado em: 2012-11
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8. RNAi-mediated knockdown of pituitary tumor- transforming gene-1 (PTTG1) suppresses the proliferation and invasive potential of PC3 human prostate cancer cells
Pituitary tumor-transforming gene-1 (PTTG1) is a proto-oncogene that promotes tumorigenesis and metastasis in numerous cell types and is overexpressed in a variety of human tumors. We have demonstrated that PTTG1 expression was up-regulated in both human prostate cancer specimens and prostate cancer cell lines. For a more direct assessment of the function of
Braz J Med Biol Res. Publicado em: 2012-11
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9. Determinação da porcentagem de espermatozóides portadores de cromossomos X e Y no sêmen sexado mediante PCR em tempo real / Determination of the proportion of X- and Y- bearing spermatozoa in bovine sexed semen by real time PCR
There are different biotechnologies to apply to increase the animal production nowadays, reproduction techniques are including. The artificial insemination (AI), for example, is a technique very used during many years ago and it is used at extensive production. The aim of this technique is to increase the numbers of pregnancies with just one ejaculation what
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 01/09/2011
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10. Impact of the tissue factor pathway inhibitor gene on apoptosis in human vascular smooth muscle cells
Tissue factor pathway inhibitor (TFPI) plays a vitally important role in the blood coagulation pathway. Recent studies indicated that TFPI induces apoptosis in vascular smooth-muscle cells (VSMCs) in animals. The present study investigated whether the TFPI gene could also induce apoptosis in human vascular smooth-muscle cells (hVSMCs). Such cells were isolat
Genetics and Molecular Biology. Publicado em: 2011
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11. Fibroblasts in programmed cell death as nuclear donors for nuclear transfer in bovines / Uso de fibroblastos em processo de morte celular programada como doadores de núcleos na técnica de transferência nuclear em bovinos
Diversos tipos celulares nas mais variadas condições têm sido usados como doadores de núcleo para a TN. Ainda não está claro se o estado fisiológico destas células afeta o posterior desenvolvimento dos embriões. Neste trabalho, testou-se a hipóese que fibroblastos bovinos em processo de MCP podem ser reprogramados na transferência nuclear. Fibrobl
Publicado em: 2009
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12. "Gene expression profiling of mantle cell lymhoma in leukemic phase with oligonucleotide microarrays" / "Análise do perfil de expressão gênica do linfoma de células do manto em fase leucêmica com microarrays de oligonucleotídeos"
Mantle cell lymphoma is associated with the translocation t(11;14)(q13;32) and overexpression of cyclin D1. Mantle cell lymphoma is predominantly disseminated at diagnosis and a frank leukemic phase is detected in one third of patients. The pre-germinal-center naive B-cells, which populate the mantle zone of the secondary lymphoid follicles, are the cells of
Publicado em: 2005