Cell Permeabilization
Mostrando 1-12 de 168 artigos, teses e dissertações.
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1. Concentrated beta-galactosidase and cell permeabilization from Saccharomyces fragilis IZ 275 for beta-galactosidase activity in the hydrolysis of lactose
Abstract The cheese whey shows an organic nutrient charge that can be used to obtain metabolites of interest by biotechnology of microorganisms. Thus, fermentative processes for enzyme production, in particular beta-galactosidase becomes feasible. The enzyme plays an important role in the biotech food industry to obtain milk and dairy products with low lacto
Food Sci. Technol. Publicado em: 27/05/2019
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2. A simple and efficient method for poly-3-hydroxybutyrate quantification in diazotrophic bacteria within 5 minutes using flow cytometry
The conventional method for quantification of polyhydroxyalkanoates based on whole-cell methanolysis and gas chromatography (GC) is laborious and time-consuming. In this work, a method based on flow cytometry of Nile red stained bacterial cells was established to quantify poly-3-hydroxybutyrate (PHB) production by the diazotrophic and plant-associated bacter
Braz J Med Biol Res. Publicado em: 16/01/2017
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3. Membrane permeabilization of colistin toward pan-drug resistant Gram-negative isolates
Abstract Pan-drug resistant Gram-negative bacteria, being resistant to most available antibiotics, represent a huge threat to the medical community. Colistin is considered the last therapeutic option for patients in hospital settings. Thus, we were concerned in this study to demonstrate the membrane permeabilizing activity of colistin focusing on investigati
Braz. J. Microbiol.. Publicado em: 2016-06
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4. Statistical investigation of Kluyveromyces lactis cells permeabilization with ethanol by response surface methodology
The aim of our study was to select the optimal operating conditions to permeabilize Kluyveromyces lactis cells using ethanol as a solvent as an alternative to cell disruption and extraction. Cell permeabilization was carried out by a non-mechanical method consisting of chemical treatment with ethanol, and the results were expressed as β-galactosidase activi
Braz. J. Microbiol.. Publicado em: 2013-12
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5. Numerical analysis of impedance spectra of yeast suspensions
Analysis of impedance spectrum of electroporated biological cell suspensions allows evaluating the state of permeabilization produced by the applied electric field. In this article we used a genetic algorithm to determine parameters of a proposed model for the impedance of suspension adjusting them numerically in order to minimize the error in relation to ex
J. Microw. Optoelectron. Electromagn. Appl.. Publicado em: 2013-12
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6. Caspase-1 activation and pore formation in murine macrophages infected with Legionella pneumophila / Ativação de caspase-1 e formação de poros em macrófagos infectados por Legionella pneumophila
Legionella pneumophila, the etiological agent of Legionnaires disease, is known to trigger pore formation in bone marrow-derived macrophages (BMMs) by mechanisms dependent on the type IVB secretion system known as Dot/Icm. Here, we used several mutants of L. pneumophila in combination with knockout mice to assess the host and bacterial factors involved in po
Publicado em: 2010
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7. Síntese de galacto-oligossacarídeos a partir de células permeabilizadas de Kluyveromyces marxianus / Galactooligosaccharides synthesis with permeabilized cells of Kluyveromyces marxianus
Galacto-oligossacarídeos (GOS) são carboidratos não digeríveis por humanos, produzidos a partir da lactose por ação da enzima ?-galactosidase. São considerados ingredientes prebióticos e possuem propriedades favoráveis à saúde dos consumidores. Este trabalho teve como objetivo o estudo da produção de GOS a partir de células permeabilizadas de K
Publicado em: 2010
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8. Toluene permeabilization differentially affects F- and P-type ATPase activities present in the plasma membrane of Streptococcus mutans
Streptococcus mutans membrane-bound P- and F-type ATPases are responsible for H+ extrusion from the cytoplasm thus keeping intracellular pH appropriate for cell metabolism. Toluene-permeabilized bacterial cells have long been used to study total membrane-bound ATPase activity, and to compare the properties of ATPase in situ with those in membrane-rich fracti
Brazilian Journal of Medical and Biological Research. Publicado em: 2008-12
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9. Efeitos da exposição crônica à poluição atmosférica particulada sobre o desenvolvimento embrionário pré-implantacional in vitro em camundongos / Effects of chronic exposure to particulate air pollution on in vitro preimplantation embryo development in mice
A thematic research project to evaluate the health effects of acute/chronic exposure to ambient air in a large urban center was developed at the Air Pollution Laboratory in the Department of Pathology at the University of São Paulo School of Medicine. Within this project a specific research line was committed to the study of the effects of this exposure on
Publicado em: 2008
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10. A mechanistic view of mitochondrial death decision pores
Mitochondria increase their outer and inner membrane permeability to solutes, protons and metabolites in response to a variety of extrinsic and intrinsic signaling events. The maintenance of cellular and intraorganelle ionic homeostasis, particularly for Ca2+, can determine cell survival or death. Mitochondrial death decision is centered on two processes: in
Brazilian Journal of Medical and Biological Research. Publicado em: 12/04/2007
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11. Estudo do efeito da fraÃÃo nÃo dialisÃvel do lÃtex de Calotropis procera (ait) R. Br em modelos experimentais de inflamaÃÃo, com Ãnfase em artrite / Study of the effect of the non-dialyzable fraction of Calotropis procera (Ait) R. Br latex in experimental models of inflammation, with emphasis in arthritis
The objective of this dissertation was to investigate the effect of the latex from Calotropis procera in experimental models of inflammation, with special focus on arthritis. The plant belongs to Asclepiadaceae family and is largely found in Northeast of Brazil. The plant latex presents clear anti-inflammatory effects, which is kept by its non-dialyzable pro
Publicado em: 2007
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12. Alterações lisossomais e indução de morte celular programada em celulas leucemicas tratadas com paladaciclo / Lysosomal alterations and programmed cell death induction in leukaemic cells treated with palladacycle
Experimental studies involving the pharmaco-toxicogical properties of new drugs are of very importance in its initial development (ERDAL et al., 2005). In this study it was evaluated the possible antileukemic effects of a new organometallic class of drugs called palladacycle ferrocene 1:2, using the K562 and Jurkat leukaemia cell lines. The cell death mechan
Publicado em: 2006