Cell Line Min6
Mostrando 13-24 de 93 artigos, teses e dissertações.
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13. Effects of polymorphonuclear leukocyte transmigration on the barrier function of cultured intestinal epithelial monolayers.
We describe a model to study the effects of polymorphonuclear leukocyte (PMN) transmigration on the intestinal epithelial barrier. Human PMN were induced to transmigrate across high resistance monolayers of a cultured human intestinal epithelial cell line (T84 cells) by chemotactic gradients produced by formyl methionyl leucyl phenylalanine (FMLP). With maxi
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14. Cloning and functional characterization of a third pituitary adenylate cyclase-activating polypeptide receptor subtype expressed in insulin-secreting cells.
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide belonging to the vasoactive intestinal polypeptide/glucagon/secretin family. It is widely distributed in the body, and a variety of biological actions have been reported. PACAP exerts its biological effects by binding to specific receptors that are coupled to GTP-binding proteins. R
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15. Pdx1 (MODY4) regulates pancreatic beta cell susceptibility to ER stress
Type 2 diabetes mellitus (T2DM) results from pancreatic β cell failure in the setting of insulin resistance. Heterozygous mutations in the gene encoding the β cell transcription factor pancreatic duodenal homeobox 1 (Pdx1) are associated with both T2DM and maturity onset diabetes of the young (MODY4), and low levels of Pdx1 accompany β cell dysfunction in
National Academy of Sciences.
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16. Infectious entry of murine retroviruses into mouse cells: evidence of a postadsorption step inhibited by acidic pH.
The entry into cells by many enveloped RNA viruses is accomplished by endocytosis and subsequent penetration of the endosomal membrane by an acidic pH-dependent fusion event. In the current study, we examined early events in the infectious entry of mouse retroviruses, using as a framework the observation that infection of a mouse tail skin cell line by the e
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17. Induction of the insulin proreceptor by hydrocortisone in cultured lymphocytes (IM-9 line).
Hydrocortisone increases the number of insulin receptors at the surface of human cultured lymphocytes (IM-9 line) without altering the degradation of the mature receptor subunits. To elucidate the effect of glucocorticoids on the biosynthesis of the insulin receptor of IM-9 cells, we preincubated cells in the presence or absence of hydrocortisone (1.4 X 10(-
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18. Simultaneous detection of CpG methylation and single nucleotide polymorphism by denaturing high performance liquid chromatography
We report here a novel method to simultaneously detect CpG methylation and single nucleotide polymorphisms (SNPs) using denaturing high performance liquid chromatography (DHPLC). PCR products of bisulfite-modified CpG islands were separated using DHPLC. BstUI digestion and DNA sequencing were used in confirmation studies. Consistent with the BstUI digestion
Oxford University Press.
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19. Rate of replication of the murine immunoglobulin heavy-chain locus: evidence that the region is part of a single replicon.
We measured the temporal order of replication of EcoRI segments from the murine immunoglobulin heavy-chain constant region (IgCH) gene cluster, including the joining (J) and diversity (D) loci and encompassing approximately 300 kilobases. The relative concentrations of EcoRI segments in bromouracil-labeled DNA that replicated during selected intervals of the
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20. Coordinate control of corticotropin, β-lipotropin, and β-endorphin release in mouse pituitary cell cultures
Hypothalamic extract stimulates the release of corticotropin (ACTH) and endorphins 2.5- to 30-fold in mouse pituitary tumor cell cultures (AtT-20/D16v line) and primary cell cultures from mouse anterior pituitary. ACTH and endorphin activities were measured by radioimmunoassay and immunoprecipitation. Pretreatment of tumor cell cultures with 1 μM dexamethas
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21. Growth of Trypanosoma cruzi in a cloned macrophage cell line and in a variant defective in oxygen metabolism.
A continuous cloned murine macrophage-like cell line, clone 16 derived from J774, has been found upon appropriate stimulation to be capable of oxidizing glucose by the hexose monophosphate shunt and producing O2- and H2O2. A variant in oxidative metabolism, clone C3C, was selected from this cell line which under similar conditions is unable to produce signif
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22. Specific receptors for leukotriene C4 on a smooth muscle cell line.
Specific receptors for leukotriene C4 (LTC4) have been identified on an intact smooth muscle cell line, DDT1 MF-2 cells derived from the Syrian hamster vas deferens. Specific [3H]LTC4 binding at a fixed input at 4 degrees C was rapid, reached a plateau at 86% of total binding at 60 min, and was reversible upon addition of excess homoligand. With incremental
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23. Overexpression of protein kinase C beta 1 enhances phospholipase D activity and diacylglycerol formation in phorbol ester-stimulated rat fibroblasts.
We are using a Rat-6 fibroblast cell line that stably overexpresses the beta 1 isozyme of protein kinase C (PKC) to study regulation of phospholipid hydrolysis by PKC. Stimulation of control (R6-C1) or overexpressing (R6-PKC3) cells with phorbol ester results in an increase in diacylglycerol (DAG) mass with no increase in inositol phosphates, indicating that
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24. Interaction of Bartonella henselae with the Murine Macrophage Cell Line J774: Infection and Proinflammatory Response
Bartonella henselae is the causative agent of cat scratch disease (CSD), a self-limiting condition characterized by a subacute regional lymphadenopathy that may develop into disseminated bartonellosis in immunocompromised subjects. Mice experimentally infected with B. henselae display typical liver and spleen granulomas rich in T cells and macrophages. So fa
American Society for Microbiology.