Cell Culture Techniques
Mostrando 13-24 de 367 artigos, teses e dissertações.
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13. Produção de carotenoides em culturas in vitro de Cleome rosea Vahl ex DC (Capparaceae) e avaliação de sua toxicidade e potencial antioxidante / Carotenoid production in vitro cultures of Cleome rosea Vahl ex DC (Capparaceae) and evaluation of toxicity and antioxidant potential.
The production and optimization of plants secondary metabolites with medicinal value have been achieved by using plant tissue culture techniques, which have showed great relevance when considering the conservation status of a species or their occurrence in degraded environments. The present study assessed the production of carotenoids in callus and cell susp
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 29/02/2012
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14. Desenvolvimento e caracterização de dispositivo de PLLA/Trietil-Citrato associado à derme suína acelular para reparação de lesões cutâneas / Development and characterization of PLLA/Triethyl citrate device associated to acellular dermal matrix to repair cutanous wounds
One of the challenges of the Tissue Engineering is to promote a better functioning of organs and tissues damaged by diseases or traumas. With target for developing a biphasic device for future regeneration and dermal application in Tissue Engineering and Regenerative Medicine, fibroblasts from VERO cell line were cultivated on a porcine acellular dermal matr
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 15/02/2012
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15. Autologous transplant: microbial contamination of hematopoietic stem cell products
Hematopoietic progenitor cells from peripheral blood (HPCPB) are commonly used for autologous and allogenic transplants in patients with most various onco-hematological diseases, and despite the utilization of sterile techniques during collection and processing of these products, bacterial contamination can occur. This study aimed to investigate the microbia
Brazilian Journal of Infectious Diseases. Publicado em: 2012-08
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16. Establishing a stem cell culture laboratory for clinical trials
Adult stem/progenitor cells are found in different human tissues. An in vitro cell culture is needed for their isolation or for their expansion when they are not available in a sufficient quantity to regenerate damaged organs and tissues. The level of complexity of these new technologies requires adequate facilities, qualified personnel with experience in ce
Revista Brasileira de Hematologia e Hemoterapia. Publicado em: 2012
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17. Estudo molecular de pestivirus em amostras de cultura celular e soro de pequenos ruminantes. / Estudo molecular de pestivirus em amostras de cultura celular e soro de pequenos ruminantes.
The Ruminants Pestiviruses are often associated with temporary and subclinical or mild clinical signs, with the largest losses related to the infection of pregnant females that can cause fetal loss and birth of persistently infected (PI) animals. The Pestivirus are distributed in many countries, including Brazil, where studies in goats and sheep are scarce.
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 2012
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18. Avaliação in vitro da adesão e proliferação de células mesenquimais do ligamento periodontal humano em diferentes superfícies de titânio
Nos últimos anos, várias pesquisas científicas em Implantodontia têm buscado alternativas que proporcionem maior rapidez e qualidade no processo de osseointegração. Diferentes métodos de tratamento podem ser utilizados para modificar as propriedades topográficas e químicas da sua superfície do titânio, a fim de otimizar as reações tecido-implant
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 04/02/2011
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19. Isolation and cultivation of fungal strains from in vitro cell cultures of two marine sponges (Porifera: Halichondrida and Haplosclerida)
Despite the large number of reports describing sponge-microbe associations, limited knowledge is available about associated fungi and their relationships with the hosts. In this work, specific fungal strains were obtained directly from in vitro sponge cell cultures (primmorphs) and single sponge cells (cytospins) and compared with those obtained from whole t
Braz. J. Microbiol.. Publicado em: 2011-12
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20. Nova técnica para contagem do número de células de blastocistos
A simplified, fast, and innovative method was developed to count the total cell number in blastocysts. Murine blastocysts (N = 195) were used in this study. They were obtained after 10h culture of initial blastocysts, compact morulae grades I and II recovered from superovulated mouse. After culture, the blastococysts were selected to test the new proposal of
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Publicado em: 2010-12
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21. Culture of human intestinal epithelial cell using the dissociating enzyme thermolysin and endothelin-3
Epithelium, a highly dynamic system, plays a key role in the homeostasis of the intestine. However, thus far a human intestinal epithelial cell line has not been established in many countries. Fetal tissue was selected to generate viable cell cultures for its sterile condition, effective generation, and differentiated character. The purpose of the present st
Brazilian Journal of Medical and Biological Research. Publicado em: 2010-05
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22. Environmentally responsive polymeric "intelligent" materials: the ideal components of non-mechanical valves that control flow in microfluidic systems
Miniaturization and commercialization of integrated microfluidic systems has had great success with the development of a wide variety of techniques in microfabrication, since they allowed their construction at a low cost and by following simple step-series procedures. However, one of the major challenges in the design of microfluidic systems is to achieve co
Brazilian Journal of Chemical Engineering. Publicado em: 2010-03
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23. Comparing two human umbilical cord mesenchymal stem cell isolation protocols
Human umbilical cord is a rich source of mesenchymal stem cells (MSCs) as reported in many studies. Many techniques are used to obtain these cells and their variations are related to the part of the tissue from which the stem cells can be extracted (umbilical vein, arteries, Wharton’s jelly or the whole umbilical cord - UC). In this sense, it is really imp
Publicado em: 2010
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24. Otimização das condições de cultivo e investigação das atividades citotóxica e antimicrobiana de metabólitos secundários do fungo endofítico Drechslera ravenelii / Optimization of the culture conditions and investigation of the cytotoxic and antimicrobial activities of the secondary metabolites from the endophytic fungus Drechslera ravenelii.
The interest in obtaining new drugs from endophytic microorganisms is growing, since this is a source still little exploited. In addition, the study of the interactions between plants and microorganisms has been widely discussed over the past few years. In this work, the endophytic fungus Drechslera ravenelii (SS33), isolated from the leaves of Smallanthus s
Publicado em: 2010