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Mostrando 13-24 de 24 artigos, teses e dissertações.
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13. In vitro lymphocyte responses of coccidioidin skin test-positive and -negative persons to coccidioidin, spherulin, and a coccidioides cell wall antigen.
The biological activity of C-ASWS, an alkali-soluble, water-soluble cell wall antigen isolated from mycelial-phase cells of Coccidioides immitis, was compared with that of a commercial coccidioidin (CDN; Cutter Laboratories); CDN-TS, a toluene-induced lysate of mycelial-phase cells; and spherulin, a spherule-derived extract of C. immitis. Lymphocytes obtaine
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14. Reactivity of alkali-soluble, water-soluble cell wall antigen of Coccidioides immitis with anti-Coccidioides immunoglobulin M precipitin antibody.
The alkali-soluble, water-soluble cell wall antigen of Coccidioides immitis (C-ASWS) mycelia and spherules was shown to react with anti-Coccidioides immunoglobulin M (IgM) precipitin antibody, both in the classical tube precipitin test and in the immunodiffusion assay for tube precipitin antibody (IDTP). The reactions obtained between C-ASWS and reference Ig
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15. Comparisons of mycelia- and spherule-derived antigens in cellular immune assays of Coccidioides immitis-infected guinea pigs.
The biological activities of four Coccidioides antigens were compared in skin test, macrophage migration inhibitory factor (MIF), and lymphocyte transformation (LT) assays fo 137 Coccidioides-infected and 20 noninfected guinea pigs. Antigens included: C-ASWS-M, the alkali-soluble, water-soluble cell wall extract from mycelia of C. immitis; C-ASWS-S, the alka
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16. Serum-mediated suppression of lymphocyte transformation responses in coccidioidomycosis.
Lymphocyte transformation (LT) responses to coccidioidin (CDN) and spherulin were suppressed in 11 (73%) of 15 patients with active coccidioidomycosis when their mononuclear cells were assayed in autologous serum as compared to serum from healthy, CDN skin test-positive subjects. Suppressed LT responses were specific for Coccidioides immitis antigens in 7 (6
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17. Direct quantification of apparent binding indices from quinidine-induced in vivo conduction delay in canine myocardium.
To characterize quantitatively the quinidine (QUIN)-induced conduction delay (CD) in vivo, canine ventricular activation times were examined with an epicardial mapping technique. A high-resolution index of normalized (N) QUIN CD, derived from all 56 recording sites, was used to quantify QUIN effect. Repetitive stimulation elicited monoexponential increases i
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18. Antisense Suppression of a (+)-δ-Cadinene Synthase Gene in Cotton Prevents the Induction of This Defense Response Gene during Bacterial Blight Infection But Not Its Constitutive Expression1[w]
In cotton (Gossypium hirsutum) the enzyme (+)-δ-cadinene synthase (CDNS) catalyzes the first committed step in the biosynthesis of cadinane-type sesquiterpenes, such as gossypol, that provide constitutive and inducible protection against pests and diseases. A cotton cDNA clone encoding CDNS (cdn1-C4) was isolated from developing embryos and functionally cha
American Society of Plant Biologists.
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19. Immunotherapy of Coccidioidomycosis
Transfer factor (TF) derived from donors with strong delayed hypersensitivity to coccidioidin (CDN) was administered to four patients with active disseminated or progressive pulmonary coccidioidomycosis. The clinical and immunologic response to TF was studied.
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20. Multiplicity of cadmium binding sites in nucleotides: X-ray evidence for the involvement of O2' and O3' as well as phosphate and N7 in inosine 5'-monophosphate.
Single-crystal X-ray methods have been used to characterize a hydrated polymeric cadmium derivative of inosine 5'-monophosphate. In the structure there are two independent cadmium atoms, one of which binds to two ribose oxygen atoms, an N7 position on a base, and to three water molecules. The second metal atom binds to a phosphate oxygen, three water molecul
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21. Comparative Study of the Cyclization Reactions of Three Bacterial Cyclomaltodextrin Glucanotransferases
The actions of cyclomaltodextrin glucanotransferases (CGTase; EC 2.4.1.19) from alkalophilic Bacillus sp. strain A2-5a (A2-5a CGTase), Bacillus macerans (Bmac CGTase), and Bacillus stearothermophilus (Bste CGTase) on amylose were investigated. All three enzymes produced large cyclic α-1,4-glucans (cycloamyloses) at the early stage of the reaction, but these
American Society for Microbiology.
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22. A comparison of prophylactic efficacy of tilmicosin and a new formulation of oxytetracycline in feedlot calves
Two replicated-pen field studies were performed under commercial feedlot conditions in western Canada to compare the administration of long-acting oxytetracycline at 30 mg/kg body weight (BW) versus tilmicosin at 10 mg/kg BW to feedlot calves upon arrival at the feedlot. Ten thousand nine hundred and eighty-nine, recently weaned, auction market derived, cros
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23. Identification of Clostridium botulinum, Clostridium argentinense, and related organisms by cellular fatty acid analysis.
On the basis of 686 analyses of 285 strains of Clostridium botulinum, Clostridium argentinense (formerly C. botulinum type G), and phenotypically related organisms, 14 cellular fatty acid (CFA) groups of toxic organisms and 6 CFA groups of nontoxic organisms were delineated. The CFA groups of toxic strains included two of type A, three of proteolytic strains
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24. Performance and Cost Evaluation of One Commercial and Six In-House Conventional and Real-Time Reverse Transcription-PCR Assays for Detection of Severe Acute Respiratory Syndrome Coronavirus
We evaluated seven reverse transcription-PCR (RT-PCR) assays, including six in-house assays and one commercial assay for the detection of severe acute respiratory syndrome coronavirus (SARS-CoV) RNA in clinical specimens. RT-PCR assays targeted different genomic regions and included three conventional assays (one nested and two non-nested) run on a conventio
American Society for Microbiology.