Cationic Lipids
Mostrando 1-12 de 55 artigos, teses e dissertações.
-
1. Evaluation of Biopolimeric Films of Cassava Starch with Incorporation of Clay Modified by Ionic Exchange and its Application as a Coating in a Fruit
Coatings technologies with biopolymeric films are effective in maintaining the chemical and sensory characteristics of several fruits. Cassava starch is an attractive biopolymeric because of its renewable characteristics and low cost, but it has a high transfer rate of water vapor. Hydrophilic compounds of organically modified montmorillonite clay by ion exc
Mat. Res.. Publicado em: 22/01/2018
-
2. Desenvolvimento de nanosistemas farmacêuticos para terapia gênica
A terapia gênica é um dos maiores desafios propostos pela pesquisa pós-genômica e se baseia na transferência de material genético a uma célula, tecido ou órgão com o intuito de curar ou melhorar o estado clínico do paciente. Em sua forma mais simples, a terapia gênica consiste na inserção de genes funcionais em células com genes defeituosos obj
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 14/03/2011
-
3. Caracterização estrutural de agregados formados pelo antifúngico anfotericina B e lipídios catiônicos: uma possível formulação farmacológica / Structural characterization of aggregates formed by the antifungal drug amphotericin B and cationic lipids: a possible pharmacological formulation
Amphotericin B (AB) is a natural antibiotic, produced by Streptomyces nodosus, with a very potent antifungal activity. It is a polyene macrolide molecule, isolated in 1953. Since then, it has been extensively used in the treatment of systemic mycotic infections. Considering AB very low solubility in aqueous medium, it has been largely used as an aqueous disp
Publicado em: 2008
-
4. A mathematical model describing the kinetic of cationic liposome production from dried lipid films adsorbed in a multitubular system
Cationic liposomes are an alternative to DNA non-viral carrier in gene therapy, promoting higher efficiency for transport and delivery into the cells. Liposome production large scale is still a challenge. Among many processes, from dried film adsorbed inside multitubular system promising due its operational simplicity. this field, engineering contributions s
Brazilian Journal of Chemical Engineering. Publicado em: 2007-12
-
5. "Propriedades estruturais de agregados anfifílicos catiônicos: interação com material genético" / structural properties of cationic amphiphilic aggregates: interaction with genetic material
Though less efficient than viral vectors, cationic lipids have been used as carriers in gene therapy. They offer several advantages over viral vectors, including the low immunogenic and inflammatory responses, the potential transfer of unlimited-size expression units, and the possibility for engineered cell-specific targeting. However, the interaction betwee
Publicado em: 2006
-
6. Adiabatic differential scanning calorimetric study of divalent cation induced DNA - DPPC liposome formulation compacted for gene delivery
Complexes between nucleic acids and phospholipid vesicles have been developed as stable non-viral gene delivery vehicles. Currently employed approach uses positively charged lipid species and a helper zwitterionic lipid, the latter being applied for the stabilization of the whole complex. However, besides problematic steps during their preparation, cationic
Brazilian Archives of Biology and Technology. Publicado em: 2004-11
-
7. Mechanism of oligonucleotide release from cationic liposomes.
We propose a mechanism for oligonucleotide (ODN) release from cationic lipid complexes in cells that accounts for various observations on cationic lipid-nucleic acid-cell interactions. Fluorescent confocal microscopy of cells treated with rhodamine-labeled cationic liposome/ fluorescein-labeled ODN (F-ODN) complexes show the F-ODN separates from the lipid af
-
8. Phosphorothioate oligodeoxyribonucleotides dissociate from cationic lipids before entering the nucleus.
Antisense oligonucleotides complementary to specific mRNA sequences are widely used inhibitors of gene expression in vitro and in vivo . In vitro cationic lipids have been demonstrated to increase the pharmacological activity of antisense oligonucleotides by increasing cellular uptake and facilitating nuclear accumulation. We have investigated the intracellu
-
9. The role of helper lipids in cationic liposome-mediated gene transfer.
In the procedure for cationic liposome-mediated transfection, the cationic lipid is usually mixed with a "helper lipid" to increase its transfection potency. The importance of helper lipids, including dioleoylphosphatidylcholine (DOPC) and phosphatidylethanolamine (dioleoyl PE), DO was examined. Freeze-fracture electron microscopy of DNA:cationic complexes c
-
10. Targeted gene transfer into hepatoma cells with lipopolyamine-condensed DNA particles presenting galactose ligands: a stage toward artificial viruses.
Optimal in vitro gene delivery with cationic lipids requires an excess of cationic charges with respect to DNA phosphates. In these conditions, in vivo delivery will be hampered by interference from cationic lipid-binding macromolecules either circulating or in the extracellular matrix. To overcome this problem, we are developing a modular transfection syste
-
11. Neutralization of β-Lysin and Cationic Bactericidal Agents by Acidic Phospholipid
Acidic phospholipids neutralized the antistaphylococcal activity of rabbit, human, and rat serum and purified rabbit β-lysin as well. Several tissue bactericidal factors and cationic antibiotics were also neutralized. The neutralizing ability of all anionic lipids tested, together with the lack of this ability for the electrostatically neutral phosphatidylc
-
12. Chemical vectors for gene delivery: a current review on polymers, peptides and lipids containing histidine or imidazole as nucleic acids carriers
DNA/cationic lipid (lipoplexes), DNA/cationic polymer (polyplexes) and DNA/cationic polymer/cationic lipid (lipopolyplexes) electrostatic complexes are proposed as non-viral nucleic acids delivery systems. These DNA-nanoparticles are taken up by the cells through endocytosis processes, but the low capacity of DNA to escape from endosomes is regarded as the m
Blackwell Publishing Ltd.