Carrot Leaves
Mostrando 13-21 de 21 artigos, teses e dissertações.
-
13. Electrical polarity in embryos of wild carrot precedes cotyledon differentiation
Endogenous electrical currents traverse embryos of a higher plant, the wild carrot Daucus carota L. Current enters the apical pole and leaves the region near the presumptive radicle in the radially symmetric globular embryo. Current also enters the exposed surfaces of incipient globular embryos. This electrical polarity precedes differentiation of vascular t
-
14. Distinct Patterns of Expression But Similar Biochemical Properties of Protein l-Isoaspartyl Methyltransferase in Higher Plants1
Protein l-isoaspartyl methyltransferase is a widely distributed repair enzyme that initiates the conversion of abnormal l-isoaspartyl residues to their normal l-aspartyl forms. Here we show that this activity is expressed in developing corn (Zea mays) and carrot (Daucus carota var. Danvers Half Long) plants in patterns distinct from those previously se
American Society of Plant Physiologists.
-
15. Bioassay and Attributes of a Growth Factor Associated with Crown Gall Tumors 1
An improved bioassay is described for a factor that promotes tumor growth which was first obtained from extracts of pinto bean leaves with crown gall tumors. Sixteen primary pinto bean leaves per sample are inoculated with sufficient Agrobacterium tumefaciens to initiate about 5 to 10 tumors per leaf and treated with tumor growth factor at day 3 after inocul
-
16. Expression and assembly of functional bacterial luciferase in plants
The luxA and luxB structural genes of Vibrio harveyi luciferase [alkanal,reduced FMN:oxygen oxidoreductase (1-hydroxylating, luminescing), EC 1.14.14.3] were introduced into a plant expression vector and transferred into tobacco and carrot cells by Agrobacterium-mediated or direct DNA transformation. Simultaneous expression of the luxA and luxB genes was mon
-
17. A Novel Protein Elicitor (PaNie) from Pythium aphanidermatum Induces Multiple Defense Responses in Carrot, Arabidopsis, and Tobacco1
A novel protein elicitor (PaNie234) from Pythium aphanidermatum (Edson) Fitzp. was purified, microsequenced, and the corresponding cDNA was cloned. The deduced amino acid sequence contains a putative eukaryotic secretion signal with a proteinase cleavage site. The heterologously expressed elicitor protein without the secretion signal of 21 amino acids (PaNie
American Society of Plant Physiologists.
-
18. Tumor induction by Agrobacterium tumefaciens prevented in Vigna sinensis seedlings systemically infected by ribonucleic acid viruses.
Cowpea (Vigna sinensis) seedlings failed to develop tumors after being inoculated with crown gall bacteria (Agrobacterium tumefaciens) if, at times earlier than 1 day later, they were inoculated on the primary leaves with a cowpea mosaic virus that systemically infects them. Inoculation with buffer or with a virus that is restricted to a localized infection,
-
19. Activity of T-DNA borders in plant cell transformation by mini-T plasmids.
By using a binary vector system, we examined the requirements for border sequences in T-DNA transformation of plant genomes. Mini-T plasmids consisting of small replicons with different extents of pTiT37 T-DNA were tested for plant tumor-inducing ability in Agrobacterium tumefaciens strain LBA4404 containing helper plasmid pAL4404 (which encodes virulence ge
-
20. Diversity among B6 strains of Agrobacterium tumefaciens.
A total of 20 laboratory substrains of Agrobacterium tumefaciens strain B6 were compared with respect to six characteristics, including 3-ketolactose production, lysogeny, octopine catabolism, tumorigenic host range, and plasmid content. Within this group of strains diversity was found for all characteristics except 3-ketolactose production. Six substrains w
-
21. Wound-Released Chemical Signals May Elicit Multiple Responses from an Agrobacterium tumefaciens Strain Containing an Octopine-Type Ti Plasmid
The vir regions of octopine-type and nopaline-type Ti plasmids direct the transfer of oncogenic T-DNA from Agrobacterium tumefaciens to the nuclei of host plant cells. Previous studies indicate that at least two genetic loci at the left ends of these two vir regions are sufficiently conserved to form heteroduplexes visible in the electron microscope. To init
American Society for Microbiology.