Cardiotoxin
Mostrando 1-12 de 13 artigos, teses e dissertações.
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1. Biochemical and toxinological characterization of Naja sumatrana (Equatorial spitting cobra) venom
The lethal and enzymatic activities of venom from Naja sumatrana (Equatorial spitting cobra) were determined and compared to venoms from three other Southeast Asian cobras (Naja sputatrix, Naja siamensis and Naja kaouthia). All four venoms exhibited the common characteristic enzymatic activities of Asiatic cobra venoms: low protease, phosphodiesterase, alkal
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2011
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2. Purificação e caracterização de uma toxina enterotóxica, citotóxina e letal produzida por amostras de Plesiomonas shigelloides isoladas de água de rio. / Purification and caracterization of an enterotoxic, cytotoxic and lethal toxin produced by Plesiomonas shigelloides isolated from river water.
Plesiomonas shigelloides is a ubiquous microorganism recognized as putative human and animal enteropathogen. Production of toxins has been related to their role in pathogenicity. At previous studies we detected a cytotoxin, named LCF (Lethal Citotoxic Factor) active on a variety of cells, causing intensive intracellular vacuolation and nuclear condensation,
Publicado em: 2008
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3. Hemolitic action of Naja naja atra cardiotoxin on erythrocytes from different animals
A comparative study on the sensitivity of erythrocytes from different vertebrate species (avian, mammalian and reptilian) to the hemolytic action caused by cardiotoxin isolated from Naja naja atra venom was carried out. Cardiotoxin was able to induce direct hemolysis in washed erythrocytes from several animals, except for llama. The EC50 values from hemolysi
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2006
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4. Isolation, purification, crystallization and preliminary crystallographic studies of sagitoxin, an oligomeric cardiotoxin from the venom of Naja naja saggitifera
Sagitoxin, a novel cardiotoxin from the venom of Naja naja saggitifera, has been successfully isolated, purified to homogeneity and crystallized.
International Union of Crystallography.
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5. Model of interaction between a cardiotoxin and dimyristoylphosphatidic acid bilayers determined by solid-state 31P NMR spectroscopy.
The interaction of cardiotoxin IIa, a small basic protein extracted from Naja mossambica mossambica venom, with dimyristoylphosphatidic acid (DMPA) membranes has been investigated by solid-state 31P nuclear magnetic resonance spectroscopy. Both the spectral lineshapes and transverse relaxation time values have been measured as a function of temperature for d
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6. Crystal structure of a snake venom cardiotoxin.
Cardiotoxin VII4 from Naja mossambica mossambica crystallizes in space group P61 (a = b = 73.9 A; c = 59.0 A) with two molecules of toxin (molecular mass = 6715 Da) in the asymmetric unit. The structure was solved by using a combination of multiple isomorphous replacement and density modification methods. Model building and least-squares refinement led to an
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7. Real-time detection of gene promoter activity: quantitation of toxin gene transcription
We have developed a new method for quantification of promoter activity in cell lines transfected with recombinant plasmids containing the reporter gene encoding chloramphenicol acetyl transferase (CAT) by real-time PCR. As the efficiency of transfection has a direct influence on the total mRNA produced, we have used the neomycin-resistance gene present
Oxford University Press.
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8. Purification and characterization of a hemolysin produced by Vibrio cholerae biotype El Tor: another toxic substance produced by cholera vibrios.
A thermolabile direct hemolysin from an El Tor cholera vibrio strain has been isolated and partially characterized as a simple protein of ca. 20,000 molecular weight. In addition to its hemolytic activity, the hemolysin is cytotoxic, cardiotoxic, and rapidly lethal. In these respects it resembles the thermostable direct hemolysin/cytotoxin/cardiotoxin/lethal
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9. The vast majority of bone-marrow-derived cells integrated into mdx muscle fibers are silent despite long-term engraftment
Bone-marrow-derived cells can contribute nuclei to skeletal muscle fibers. However, serial sectioning of muscle in mdx mice implanted with GFP-labeled bone marrow reveals that only 20% of the donor nuclei chronically incorporated in muscle fibers show dystrophin (or GFP) expression, which is still higher than the expected frequency of “revertant” fibers,
National Academy of Sciences.
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10. Postsynaptic α-Neurotoxin Gene of the Spitting Cobra, Naja naja sputatrix: Structure, Organization, and Phylogenetic Analysis
The venom of the spitting cobra, Naja naja sputatrix contains highly potent α-neurotoxins (NTXs) in addition to phospholipase A2 (PLA2) and cardiotoxin (CTX). In this study, we report the complete characterization of three genes that are responsible for the synthesis of three isoforms of α-NTX in the venom of a single spitting cobra. DNA amplification by l
Cold Spring Harbor Laboratory Press.
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11. Mouse Pop1 Is Required for Muscle Regeneration in Adult Skeletal Muscle
Popeye (Pop) genes are a novel gene family encoding putative transmembrane proteins predominantly present in striated and smooth muscle cells. In this study, a null mutation of Pop1 was generated by replacing the first coding exon of the Pop1 gene with the lacZ reporter gene. Homozygous mice lacking Pop1 were fertile and had a normal life span without any ap
American Society for Microbiology.
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12. Cardiotrophin-1 Maintains the Undifferentiated State in Skeletal Myoblasts*
Skeletal myogenesis is potently regulated by the extracellular milieu of growth factors and cytokines. We observed that cardiotrophin-1 (CT-1), a member of the interleukin-6 (IL-6) family of cytokines, is a potent regulator of skeletal muscle differentiation. The normal up-regulation of myogenic marker genes, myosin heavy chain (MyHC), myogenic regulatory fa
American Society for Biochemistry and Molecular Biology.