Camptotheca
Mostrando 1-6 de 6 artigos, teses e dissertações.
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1. Antitumor potential of flavonoids derived from northeastern brazilian plants: preliminary studies on structure-cytotoxic activity relationship / Potencial antitumoral de flavonóides isolados de plantas do nordeste brasileiro: estudos preliminares da relação estrutura-atividade citotóxica
In searching for anticancer compounds derived from plant sources, 18 flavonoids were assayed for their cytotoxic potentials and the results were compared for structure-activity relationship purposes. The flavonoid group was subdivided in flavones and pterocarpans. The cytotoxic activity was initially evaluated on tumor cell lines, through the MTT assay, and
Publicado em: 2005
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2. Expression of a 3-hydroxy-3-methylglutaryl coenzyme A reductase gene from Camptotheca acuminata is differentially regulated by wounding and methyl jasmonate.
We have isolated a gene, hmg1, for 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) from Camptotheca acuminata, a Chinese tree that produces the anti-cancer monoterpenoid indole alkaloid camptothecin (CPT). HMGR supplies mevalonate for the synthesis of the terpenoid component of CPT as well as for the formation of many other primary and secondary metab
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3. Tissue-Specific Expression of the β-Subunit of Tryptophan Synthase in Camptotheca acuminata, an Indole Alkaloid-Producing Plant1
Camptothecin is an anticancer drug produced by the monoterpene indole alkaloid pathway in Camptotheca acuminata. As part of an investigation of the camptothecin biosynthetic pathway, we have cloned and characterized a gene from C. acuminata encoding the β-subunit of tryptophan (Trp) synthase (TSB). In C. acuminata TSB provides Trp for both protein syn
American Society of Plant Physiologists.
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4. Characterization of a mammalian mutant with a camptothecin-resistant DNA topoisomerase I.
DNA topoisomerase I was purified to near homogeneity from a clonal line of human lymphoblastic leukemia cells, RPMI 8402, that is resistant to camptothecin, a cytotoxic alkaloid from Camptotheca acuminata, and compared with that of the parent wild-type cells. As assayed by relaxation of the supercoiled plasmid DNA and by formation of enzyme-linked DNA breaks
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5. Bcmfs1, a Novel Major Facilitator Superfamily Transporter from Botrytis cinerea, Provides Tolerance towards the Natural Toxic Compounds Camptothecin and Cercosporin and towards Fungicides
Bcmfs1, a novel major facilitator superfamily gene from Botrytis cinerea, was cloned, and replacement and overexpression mutants were constructed to study its function. Replacement mutants showed increased sensitivity to the natural toxic compounds camptothecin and cercosporin, produced by the plant Camptotheca acuminata and the plant pathogenic fungus Cerco
American Society for Microbiology.
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6. Interaction of an alkylating camptothecin derivative with a DNA base at topoisomerase I-DNA cleavage sites.
DNA topoisomerase I (top1) is a ubiquitous nuclear enzyme. It is specifically inhibited by camptothecin, a natural product derived from the bark of the tree Camptotheca acuminata. Camptothecin and several of its derivatives are presently in clinical trial and exhibit remarkable anticancer activity. The present study is a further investigation of the molecula