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Mostrando 13-24 de 83 artigos, teses e dissertações.
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13. Twenty-four hour presence of physicians in the ICU
Intensive-care units (ICUs) must be utilised in the most efficient way. Greater input of intensivists leads to better outcomes and more efficient use of resources. 'Closed' ICUs operate as functional units with a competent on-site team and their own management under the supervision of a full-time intensivist directly responsible for the treatment. Twenty-fou
BioMed Central.
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14. Telomeric site position heterogeneity in macronuclear DNA of Paramecium primaurelia.
In Paramecium primaurelia, the macronuclear gene encoding the G surface protein is located near a telomere. In this study, multiple copies of this telomere have been isolated and the subtelomeric and telomeric regions of some of them have been sequenced. The telomeric sequences consist of tandem repeats of the hexanucleotides C4A2 or C3A3. We show that the l
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15. Novel internal promoter/enhancer of HTLV-I for Tax expression.
The transcription initiation signals in retroviruses lie within the long terminal repeat (LTR) sequences. We have found a new transcriptional promoter in a central portion of the genome of human T-cell leukaemia virus type I (HTLV-I). The transcription start site is located just upstream to the ATG codon of the transcriptional trans-activator molecule, tax p
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16. Evidence that introns arose at proto-splice sites.
The unexpected discovery of introns raised many questions about gene evolution. We provide evidence that actin and tubulin introns were gained between the G and R of the conserved coding sequence C/AAGR that is known to flank introns in general and which we call a proto-splice site. We conclude that the tubulin and actin introns are less ancient than the cod
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17. Rearrangement of immunoglobulin gamma 1-chain gene and mechanism for heavy-chain class switch.
Cloning and nucleotide sequence determination suggest that the rearranged gamma 1-chain gene in a gamma 1-chain-producing myeloma a-pears to be formed by the recombination between the 5' flanking regions of the gamma 1- and mu-chain genes of undifferentiated cells. The recombination site is distinct from the putative J region and is a novel region that we ca
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18. Suppression of spectinomycin resistance in a mutant of Escherichia coli K-12.
A mutant of Escherichia coli has been isolated which exhibits partial suppression of spectinomycin resistance. The site of mutation is in the streptomycin (strA) region and is closely linked to the spcA gene. However, this gene, which we propose to call mod, is phenotypically distinguishable from both the neomycin-kanamycin (nek) and the ribosomal ambiguity
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19. A cosmid vector that facilitates restriction enzyme mapping.
We describe the construction and use of a cosmid vector, loric, which is derived from the phage lambda origin of replication and appears to be more stable than ColE1-derived cosmids. Loric recombinants can be efficiently packaged in vivo to yield 100-300 micrograms of DNA per liter that is linear and has single-stranded cos ends. We call such molecules "phos
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20. Localization of Antibody-Combining Sites within the Variable Portions of Heavy and Light Chains
The Fab′-fragment of a mouse IgA-myeloma (protein-315) was split by pepsin to yield a smaller fragment that retained the anti-2,4-dinitrophenyl activity of the intact protein. This fragment, which we call Fv, has a molecular weight of about 30,000 (half that of Fab′), and is composed of two polypeptide chains (molecular weight 14,000) held together by no
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21. Site-directed mutagenesis reveals a liver transcription factor essential for the albumin transcriptional enhancer.
A method based on the polymerase chain reaction is described for constructing a clustered set of basepair changes, deletions, or insertions at any site on a DNA fragment. Advantages of the procedure are that virtually every product has the desired sequence alteration and that only a single round of polymerase chain reaction is required. We used this method t
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22. Chromosomal location of a gene (nmpA) involved in expression of a major outer membrane protein in Escherichia coli.
The phenotypic expression of protein E, a recently described major outer membrane protein, is associated with a mutation at a locus on the Escherichia coli chromosome that we call nmpA. nmpA is located between rbsK and uncA at 82.7 min on the E. coli linkage map. The nmpA locus is also the site of the mutations which lead to the formation of major outer memb
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23. DNA-binding specificity and dimerization of the DNA-binding domain of the PEND protein in the chloroplast envelope membrane
The PEND protein is a DNA-binding protein in the inner envelope membrane of a developing chloroplast, which may anchor chloroplast nucleoids. Here we report the DNA-binding characteristics of the N-terminal basic region plus leucine zipper (bZIP)-like domain of the PEND protein that we call cbZIP domain. The basic region of the cbZIP domain diverges sig
Oxford University Press.
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24. Illegitimate replication of linear hepadnavirus DNA through nonhomologous recombination.
Linear hepadnavirus DNA in primary hepatocyte cultures efficiently participates in intra- and intermolecular nonhomologous recombination at its ends. The products of this recombination are (i) monomeric covalently closed circular DNAs (cccDNAs) with deletions and insertions around the site of joining and (ii) oligomeric forms in which monomers are joined nea