Bulk Segregant Analysis
Mostrando 1-12 de 14 artigos, teses e dissertações.
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1. Rbc2, a new dominant gene for resistance of soybean to Cowpea mild mottle virus: Inheritance and mapping
Abstract The objective of this work was to study the inheritance of soybean resistance to Soybean Steam Necrosis (SSN) and to map the resistance gene(s), using an F2 population from the cross between the soybean cultivars BRS 133 (resistant) and CD 206 (susceptible). A phenotypic evaluation was performed under controlled conditions, in a greenhouse. In a pop
Crop Breed. Appl. Biotechnol.. Publicado em: 2018-04
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2. Mapping and allelic sequencing of a long sterile lemma trait in rice
ABSTRACT Some outer floral organs are unique in gramineous plants, like the sterile lemma and rudimentary glume in rice. However, their development mechanisms are still poorly understood. In this study, we used 4 mutants with long sterile lemma (LSL), named JF11, JF12, JF13 and JNY-7, to be crossed with Aijiaonante (AJNT) and Nipponbare (NIP), respectively.
Bragantia. Publicado em: 15/05/2017
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3. Análise morfológica, bioquímica e genética do brilho do tegumento em variedades de feijoeiro (Phaseolus vulgaris L.) / Morphological, biochemical and genetic analysis of seed coat shininess in common bean varieties (Phaseolus vulgaris L.)
Several characteristics interfere in acceptability of common bean varieties (Phaseolus vulgaris L.) by consumers, like seed coat shininess. Generally, varieties with shiny seed coat are rejected by consumers due to their difficult cooking. However, shininess could confer resistance to insects attak and also pathogens to the seed, giving an advantage for this
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 03/06/2011
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4. Identificação rápida de marcadores SSR polimórficos em Coffea arabica.
2010
In.: CONGRESSO BRASILEIRO DE GENÉTICA. Publicado em: 2011
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5. Identificação rápida de marcadores SSR polimórficos em Coffea arabica.
2010
In.: CONGRESSO BRASILEIRO DE GENÉTICA. Publicado em: 2011
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6. AFLP marker linked to water-stress-tolerant bulks in barley (Hordeum vulgare L.)
The amplified fragment length polymorphism (AFLP) assay is an efficient method for the identification of molecular markers, useful in the improvement of numerous crop species. Bulked Segregant Analysis (BSA) was used to identify AFLP markers associated with water-stress tolerance in barley, as this would permit rapid selection of water-stress tolerant genoty
Genetics and Molecular Biology. Publicado em: 2003
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7. RAPD markers linked to a block of genes conferring rust resistance to the common bean
A ferrugem, causada pelo fungo Uromyces appendiculatus, pode ocasionar perdas significativas na produtividade do feijoeiro-comum (Phaseolus vulgaris L.). Marcadores RAPD fortemente ligados a genes de resistência à ferrugem podem ser usados em programas de melhoramento para auxiliar no desenvolvimento de cultivares resistentes. Nesse sentido, o objetivo do
Genetics and Molecular Biology. Publicado em: 2000-06
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8. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations.
We developed bulked segregant analysis as a method for rapidly identifying markers linked to any specific gene or genomic region. Two bulked DNA samples are generated from a segregating population from a single cross. Each pool, or bulk, contains individuals that are identical for a particular trait or genomic region but arbitrary at all unlinked regions. Th
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9. Rapid Array Mapping of Circadian Clock and Developmental Mutations in Arabidopsis1
Classical forward genetics, the identification of genes responsible for mutant phenotypes, remains an important part of functional characterization of the genome. With the advent of extensive genome sequence, phenotyping and genotyping remain the critical limiting variables in the process of map-based cloning. Here, we reduce the genotyping problem by hybrid
American Society of Plant Biologists.
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10. Genetic Mapping of Quantitative Trait Loci Controlling Growth and Wood Quality Traits in Eucalyptus Grandis Using a Maternal Half-Sib Family and Rapd Markers
Quantitative trait loci (QTL) mapping of forest productivity traits was performed using an open pollinated half-sib family of Eucalyptus grandis. For volume growth, a sequential QTL mapping approach was applied using bulk segregant analysis (BSA), selective genotyping (SG) and cosegregation analysis (CSA). Despite the low heritability of this trait and the h
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11. Microarray-based rapid cloning of an ion accumulation deletion mutant in Arabidopsis thaliana
Here we describe the development of a microarray-based mapping strategy to rapidly isolate deletion mutant genes. The presented approach is particularly useful for mapping mutant genes that are difficult to phenotype. This strategy uses masking bulk segregant analysis to mask unrelated deletions, thus allowing identification of target deletions by microarray
National Academy of Sciences.
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12. Large-Scale Identification of Single-Feature Polymorphisms in Complex Genomes
We have developed a high-throughput genotyping platform by hybridizing genomic DNA from Arabidopsis thaliana accessions to an RNA expression GeneChip (AtGenome1). Using newly developed analytical tools, a large number of single-feature polymorphisms (SFPs) were identified. A comparison of two accessions, the reference strain Columbia (Col) and the strain Lan
Cold Spring Harbor Laboratory Press.