Brucella Canis
Mostrando 25-36 de 58 artigos, teses e dissertações.
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25. Differentiation of Brucella canis from Other Brucellae by Gas Chromatography
Gas chromatographic techniques allow for differentiation between a strain of Brucella canis and strains of other brucellae.
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26. Immunochemical characterization of rough Brucella lipopolysaccharides.
Lipopolysaccharides (LPS) were extracted from rough strains of Brucella abortus and Brucella melitensis and from strains of the naturally occurring rough species Brucella ovis and Brucella canis. Brucella rough lipopolysaccharides (R-LPS) were readily distinguished from Brucella smooth lipopolysaccharides (S-LPS) and enterobacterial R-LPS, by their chemical,
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27. Microtiter Plate Agglutination Test for Brucella canis Antibodies
A micro-agglutination test for the antibodies to Brucella canis produced similar results to those obtained with the standard tube agglutination method in human and canine sera. The micromethod does provide an economical means of screening sera for the presence of antibodies.
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28. Endotoxic activity of rough organisms of Brucella species.
A rough-specific antigen extracted from the rough species Brucella ovis and lipopolysaccharide extracted from smooth Brucella abortus demonstrated equivalent levels of activity in tests for mouse lethality and limulus lysate clotting activity. Acetone-extracted whole cells of B. ovis and of B. canis and of a rough mutant of B; abortus had the same toxicity f
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29. Taxonomic Position in the Genus Brucella of the Causative Agent of Canine Abortion
The gram-negative organism causing abortion in dogs was examined in parallel with cultures representative of the Brucella species and with Bordetella bronchiseptica. The organism fits into the genus Brucella and most closely resembles B. suis on the basis of its growth characteristics. It is of rough colonial morphology and is agglutinated by antisera prepar
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30. Seroepidemiological investigation of Brucella canis antibodies in different human population groups.
A study was conducted to determine the prevalence of Brucella canis antibodies in specified groups based on their exposure to dogs. The method used was a microtiter technique, and the presence of antibodies at a 1:12 or greater dilution of serum was considered a positive test. Eleven (5.7%) of the newborn infants had evidence of maternal antibodies, and 67.8
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31. Limited Genetic Diversity of Brucella spp.
Multilocus enzyme electrophoresis (MLEE) of 99 Brucella isolates, including the type strains from all recognized species, revealed a very limited genetic diversity and supports the proposal of a monospecific genus. In MLEE-derived dendrograms, Brucella abortus and a marine Brucella sp. grouped into a single electrophoretic type related to Brucella neotomae a
American Society for Microbiology.
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32. Binding of bacteria from the genus Brucella to human B lymphocytes.
In previous studies, we have shown that various lymphocyte subpopulations bind different strains of bacteria of different genera and species. Among these bacteria was a strain of Brucella melitensis which bound to all human B lymphocytes. To determine whether the binding of B. melitensis to human B lymphocytes was strain, species, or genus characteristic, we
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33. Occurrence of Quinovosamine in Lipopolysaccharides of Brucella Species 1
Lipopolysaccharides obtained from Brucella abortus, B. melitensis, and B. suis, but not B. canis, were found to contain amino sugars identified as glucosamine and quinovosamine by cation exchange and thin-layer cellulose chromatography and ninhydrin degradation.
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34. Purification of a Brucella canis cell wall antigen by using immunosorbent columns and use of the antigen in enzyme-linked immunosorbent assay for specific diagnosis of canine brucellosis.
A cell wall antigen of Brucella canis was purified by immunosorbent columns. The antigen contained two proteins of 30 and 28 kilodaltons and a polysaccharide exhibiting a 12-kilodalton band upon 12.5% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Antibody to the purified antigen, which specifically reacted with the polysaccharide, was used as th
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35. Use of Amplified Fragment Length Polymorphism To Identify and Type Brucella Isolates of Medical and Veterinary Interest
Amplified fragment length polymorphism (AFLP) is a whole-genome fingerprinting method that relies on the selective PCR amplification of restriction fragments. The potential of this approach for the discrimination of Brucella isolates at the species and intraspecies level was assessed. A number of different combinations of restriction enzymes and selective pr
American Society for Microbiology.
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36. Molecular Characterization of Brucella Strains Isolated from Marine Mammals
Recently, gram-negative bacteria isolated from a variety of marine mammals have been identified as Brucella species by conventional phenotypic analysis. This study found the 16S rRNA gene from one representative isolate was identical to the homologous sequences of Brucella abortus, B. melitensis, B. canis, and B. suis. IS711-based DNA fingerprinting of 23 is
American Society for Microbiology.