Bradyrhizobia
Mostrando 13-24 de 37 artigos, teses e dissertações.
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13. Symbiotic Effectiveness of Indigenous Soybean Bradyrhizobia as Related to Serological, Morphological, Rhizobitoxine, and Hydrogenase Phenotypes †
A Collection of 360 isolates of Bradyrhizobium japonicum was developed from soybean (Glycine max [L.] Merrill) nodules taken from 18 locations in Delaware. The isolates were characterized serologically with an enzyme-linked immunosorbent assay, morphologically by colony type on yeast extract-mannitol agar, and for production of rhizobitoxine symptoms with so
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14. Effect of Bradyrhizobium photosynthesis on stem nodulation of Aeschynomene sensitiva
Some leguminous species of the genus Aeschynomene are specifically stem-nodulated by photosynthetic bradyrhizobia. To study the effect of bacterial photosynthesis during symbiosis, we generated a photosynthesis-negative mutant of the Bradyrhizobium sp. strain ORS278 symbiont of Aeschynomene sensitiva. The presence of a functional photosynthetic unit in
The National Academy of Sciences.
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15. Hydrogen Oxidation by the Host-Controlled Uptake Hydrogenase Phenotype of Bradyrhizobium japonicum in Symbiosis with Soybean Host Plants
Symbioses between uptake hydrogenase host-regulated (Hup-hr) phenotypes of Bradyrhizobium japonicum and exotic, agronomically unadapted soybean germ plasm were examined for expression of uptake hydrogenase activity. Determinations for hydrogen evolution and uptake hydrogenase activity identified five plant introduction (PI) lines which formed hydrogen-oxidiz
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16. Cell-associated oligosaccharides of Bradyrhizobium spp.
We report the initial characterization of the cell-associated oligosaccharides produced by four Bradyrhizobium strains: Bradyrhizobium japonicum USDA 110, USDA 94, and ATCC 10324 and Bradyrhizobium sp. strain 32H1. The cell-associated oligosaccharides of these strains were found to be composed solely of glucose and were predominantly smaller than the cyclic
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17. Serological Relatedness of Rhizobium fredii to Other Rhizobia and to the Bradyrhizobia
Several isolates of Rhizobium fredii were examined for their serological relatedness to each other, to Bradyrhizobium japonicum, and to other fast- and slow-growing rhizobia. Immunofluorescence, agglutination, and immunodiffusion analyses indicated that R. fredii contains at least three separate somatic serogroups, USDA 192, USDA 194, and USDA 205. There was
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18. New Method of Denitrification Analysis of Bradyrhizobium Field Isolates by Gas Chromatographic Determination of 15N-Labeled N2
To evaluate the denitrification abilities of many Bradyrhizobium field isolates, we developed a new 15N-labeled N2 detection methodology, which is free from interference from atmospheric N2 contamination. 30N2 (15N15N) and 29N2 (15N14N) were detected as an apparent peak by a gas chromatograph equipped with a thermal conductivity detector with N2 gas having n
American Society for Microbiology.
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19. Lotus corniculatus nodulation specificity is changed by the presence of a soybean lectin gene
Plant lectins have been implicated as playing an important role in mediating recognition and specificity in the Rhizobium-legume nitrogen-fixing symbiosis. To test this hypothesis, we introduced the soybean lectin gene Le1 either behind its own promoter or behind the cauliflower mosaic virus 35S promoter into Lotus corniculatus, which is nodulated by R. loti
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20. The Aquatic Budding Bacterium Blastobacter denitrificans Is a Nitrogen-Fixing Symbiont of Aeschynomene indica
Blastobacter spp. are freshwater bacteria that form rosette structures by cellular attachment to a common base. Comparative analyses of ribosomal 16S rRNA gene and internally transcribed spacer region sequences indicated that B. denitrificans is a member of the α-subdivision of Proteobacteria. Among the α-Proteobacteria, B. denitrificans was related to a c
American Society for Microbiology.
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21. K+ regulates bacteroid-associated functions of Bradyrhizobium
Cowpea Bradyrhizobium 32H1 cells, when grown under 0.2% O2, synthesize nitrogenase, as well as a methylammonium (ammonium) transport system and an electrogenic K+/H+ antiporter. This effect was seen in growth medium containing 8-12 mM K+ but not with 50 μM K+. Addition of K+ to cells growing under low O2 tensions in low-K+ medium led to various phenotypic p
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22. The Occurrence of Phytoferritin and Its Relationship to Effectiveness of Soybean Nodules 1
Polyacrylamide gel electrophoresis and electron microscopy revealed that accumulation of iron-protein in soybean nodules is influenced by nodule age, mutation in bradyrhizobia, and rhizobial/bradyrhizobial strain-soybean cultivar interactions. Iron-protein concentrations (micrograms per milligram protein) were inversely related to heme concentrations (nanomo
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23. Three unusual modifications, a D-arabinosyl, an N-methyl, and a carbamoyl group, are present on the Nod factors of Azorhizobium caulinodans strain ORS571.
Azorhizobium caulinodans strain ORS571 is a symbiont of the tropical legume Sesbania rostrata. Upon nod gene induction with naringenin, strain ORS571 secretes into the culture medium Nod factors that morphologically change the host plant--in particular, deformed root hairs (Hai/Had) and meristematic foci are formed at the basis of lateral roots. The latter i
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24. Enzymes of the beta-ketoadipate pathway are inducible in Rhizobium and Agrobacterium spp. and constitutive in Bradyrhizobium spp.
Protocatechuate is a universal growth substrate for members of the family Rhizobiaceae, and these bacteria utilize the aromatic compound via the beta-ketoadipate pathway. This report describes transcriptional controls exercised by different subgroups of the Rhizobiaceae over five enzymes that catalyze consecutive reactions in the pathway: protocatechuate oxy