Bovine Paratuberculosis
Mostrando 1-12 de 54 artigos, teses e dissertações.
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1. Detection of Mycobacterium avium subsp. paratuberculosis in bovine milk from the state of Pernambuco, Brazil
Abstract The aim of this study was to detect the IS900 region of Mycobacterium avium subsp. paratuberculosis (MAP) in bovine milk samples using real-time polymerase chain reaction (qPCR) and conventional PCR, and to study the agreement between these tests. A total of 121 bovine milk samples were collected from herds considered positive for MAP, from the Stat
Braz. J. Microbiol.. Publicado em: 2017-03
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2. Bovine response to lipoarabinomannan vaccination and challenge with Mycobacterium paratuberculosis
This study aimed to evaluate the immune response in bovines following immunization with a mycobaterial Lipoarabinomannan extract (LAMe) and the effect of Map challenge. LAMe vaccine induced specific antibody levels that diminished after the challenge and affected Map excretion at least for 100 days thereafter.
Braz. J. Microbiol.. Publicado em: 2013
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3. Aspectos clínico-patológicos da paratuberculose em rebanho bovino leiteiro no município de Rio Claro, RJ. / Clinic-pathological aspects of paratuberculosis in dairy cattle in Rio Claro county, Rio de Janeiro State.
The epidemic and clinic-pathological aspects of paratuberculois in a dairy cattle herd in the Rio Claro county, southern Rio de Janeiro region, are described. In the years 2006-2009, eight adult cows presented chronic-intermittent diarrhea, chronic weight loss and normal appetite. At necropsy, the subserosal lymphatics were proeminent and dilated, mesenteric
Publicado em: 2010
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4. Aspectos epidemiológicos da paratuberculose bovina no Rio Grande do Sul / Epidemiologic aspects of bovine paratuberculosis in Rio Grande do Sul – RS
Descrevem-se os achados clínicos e patológicos da paratuberculose em uma criação intensiva de bovinos de leite no municí pio de Capela de Santana, RS. Os sinais clínicos foram observados em oito de um total de 345 bovinos, consistindo em diarréia crônica refratária aos tratamentos, emagrecimento progressivo e queda na produção de leite. As princip
Publicado em: 2007
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5. AhpC, AhpD, and a Secreted 14-Kilodalton Antigen from Mycobacterium avium subsp. paratuberculosis Distinguish between Paratuberculosis and Bovine Tuberculosis in an Enzyme-Linked Immunosorbent Assay
Sera from cattle naturally infected with Mycobacterium avium subsp. paratuberculosis (n = 56) and naturally (n = 4) and experimentally (n = 8) infected with Mycobacterium bovis were tested for the presence of antibodies against paratuberculosis antigens. An enzyme-linked immunosorbent assay (ELISA) was established based on absorption of M. avium subsp. parat
American Society for Microbiology.
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6. Use of Hoechst 33342 Staining To Detect Apoptotic Changes in Bovine Mononuclear Phagocytes Infected with Mycobacterium avium subsp. paratuberculosis
Mycobacterium avium subsp. paratuberculosis is an intracellular pathogen of macrophages that causes a chronic enteritis (Johne's disease) in ruminants. The purpose of this study was to determine whether M. avium subsp. paratuberculosis infection causes apoptosis in bovine monocytes. Using Hoechst 33342 staining, we observed increased numbers of apoptotic mon
American Society for Microbiology.
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7. Effects of gamma interferon and nitric oxide on the interaction of Mycobacterium avium subsp. paratuberculosis with bovine monocytes.
In this study, we examined the effects of recombinant bovine gamma interferon (rIFN-gamma) and nitric oxide (NO) on the interaction of M. avium subsp. paratuberculosis with bovine monocytes. Monocytes pretreated with rIFN-gamma exhibited slightly increased phagocytosis of M. avium subsp. paratuberculosis and modest inhibition of the intracellular growth of t
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8. Ingestion and intracellular growth of Mycobacterium paratuberculosis within bovine blood monocytes and monocyte-derived macrophages.
Cellular immunity is thought to be of major importance in resistance to infection with Mycobacterium paratuberculosis, the causative agent of Johne's disease of ruminants. The results of this study clarify the influence of bovine mononuclear phagocyte maturation on the ingestion and intracellular survival of M. paratuberculosis in vitro. Optimal phagocytosis
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9. Immunohistochemical distribution of ferritin, lactoferrin, and transferrin in granulomas of bovine paratuberculosis.
Granulomatous lesions of bovine paratuberculosis contained ferritin, lactoferrin, and a small amount of transferrin, as demonstrated by the immunohistochemical method. Macrophages in the normal bovine ileum did not contain lactoferrin and transferrin; however, ferritin was found in individual macrophages of Peyer's patches. These results may help elucidate t
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10. Cytokine regulation of the intracellular growth of Mycobacterium paratuberculosis in bovine monocytes.
In this study we examined the influence of various crude and recombinant cytokines on the ingestion and intracellular survival of Mycobacterium paratuberculosis within bovine monocytes and monocyte-derived macrophages. Cytokine pretreatment had little effect on the ingestion of M. paratuberculosis by bovine monocytes and macrophages. Monocytes that were cont
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11. Experimental infection of severe combined immunodeficient beige mice with Mycobacterium paratuberculosis of bovine origin.
Severe combined immunodeficient beige mice were inoculated orally and intraperitoneally with a bovine strain of Mycobacterium paratuberculosis to explore their potential as laboratory animal models in the study of paratuberculosis (Johne's disease). Control animals were similarly inoculated with heat-killed M. paratuberculosis. In the mice inoculated intrape
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12. Molecular Epidemiology of Mycobacterium avium subsp. paratuberculosis: Evidence for Limited Strain Diversity, Strain Sharing, and Identification of Unique Targets for Diagnosis
The objectives of this study were to understand the molecular diversity of animal and human strains of Mycobacterium avium subsp. paratuberculosis isolated in the United States and to identify M. avium subsp. paratuberculosis-specific diagnostic molecular markers to aid in disease detection, prevention, and control. Multiplex PCR of IS900 integration loci (M
American Society for Microbiology.