Bovine Embryos
Mostrando 1-12 de 99 artigos, teses e dissertações.
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1. Efficacy and limitations of different approaches to anticipate the diagnosis of pregnancy in cattle
RESUMO O estudo foi planejado para correlacionar exames ultrassonográficos e sorológicos realizados para o diagnóstico precoce (20 a 30d) de gestação. Cento e vinte (n= 120) receptoras bovinas foram sincronizadas (estro=D0), e embriões frescos produzidos in vitro foram transferidos em tempo fixo (TETF, D7). No experimento 1 (n= 46), o diagnóstico de g
Arq. Bras. Med. Vet. Zootec.. Publicado em: 13/12/2019
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2. Oocytes, embryos and pluripotent stem cells from a biomedical perspective
Abstract The veterinary and animal science professions are rapidly developing and their inherent and historical connection to agriculture is challenged by more biomedical and medical directions of research. While some consider this development as a risk of losing identity, it may also be seen as an opportunity for developing further and more sophisticated co
Anim. Reprod.. Publicado em: 28/11/2019
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3. Use of two new formulations as bovine embryo manipulation solution
Abstract This study aimed to evaluate the effect of two Embryo Manipulation Solutions (EMS and EMS supplemented) in maintenance of the viability of embryos, initially using structures derived from mice (first phase). Next, the efficiency of these solutions in routines of bovine embryo transfer was evaluated (second stage). Mice embryos were used in the stage
Anim. Reprod.. Publicado em: 25/11/2019
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4. Effect of Rolipram on in vitro maturation, gene expression and embryonic development in bovines
RESUMO O objetivo deste trabalho foi avaliar o efeito do rolipram durante a maturação de oócitos bovinos, expressão gênica e embriões produzidos in vitro. Os ovários bovinos foram coletados no matadouro. Os COCs foram selecionados e divididos em cinco grupos: controle 0 tempo; controle: MIV por 24 horas; tratamentos rolipram com bloqueio MIV por 24 ho
Arq. Bras. Med. Vet. Zootec.. Publicado em: 28/10/2019
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5. Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content
ABSTRACT This study evaluated the effect of the addition of conjugated linoleic acid (CLA) to in vitro culture on viability, lipid content, and cryoresistance of bovine embryos at different in vitro culture times. Cumulus oocyte complexes (N = 974) were maturated in vitro for 22 h. In vitro fecundation ensued for 18 h. Viable zygotes were cultivated in vitro
R. Bras. Zootec.. Publicado em: 30/09/2019
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6. Antioxidant activity of oily extract obtained from Lippia origanoides improves the quality of bovine embryos produced in vitro
RESUMO O objetivo desse estudo foi avaliar a suplementação de meio de cultura de embriões com antioxidante obtido do extrato oleoso da Lippia origanoides no desenvolvimento e na qualidade de blastocistos produzidos in vitro. Oócitos coletados de ovários de matadouros foram maturados e fertilizados in vitro segundo procedimento laboratorial padrão. Zigo
Arq. Bras. Med. Vet. Zootec.. Publicado em: 14/06/2019
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7. Aspects of energetic substrate metabolism of in vitro and in vivo bovine embryos
Although the metabolism of early bovine embryos has not been fully elucidated, several publications have addressed this important issue to improve culture conditions for cattle reproductive biotechnologies, with the ultimate goal of producing in vitro embryos similar in quality to those developing in vivo. Here, we review general aspects of bovine embryo met
Braz J Med Biol Res. Publicado em: 23/01/2015
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8. Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro
This study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and classified according to meiotic progression and migration of cortical granules. In
R. Bras. Zootec.. Publicado em: 2014-03
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9. CARACTERIZAÇÃO MOLECULAR DOS COMPONENTES DO SISTEMA ANGIOTENSINA-(1-7) DURANTE A DIVERGÊNCIA FOLICULAR E EXPRESSÃO DE GENES DE REPARO DA FITA DUPLA DE DNA EM EMBRIÕES BOVINOS / MOLECULAR CHARACTERIZATION OF THE ANGIOTENSIN-(1-7) SYSTEM COMPONENTS DURING FOLLICULAR DEVIATION AND EXPRESSION OF DNA DOUBLE-STRANDED REPAIR GENES IN BOVINE EMBRYOS
The first study characterized the expression of MAS receptor and key enzymes for Ang-(1-7) production, such as, ACE2, NEP and PEP during follicular development. Furthermore, the regulation of local Ang1-7 system was evaluated after the intrafollicular injection of fulvestrant (an estradiolreceptor inhibitor) in the dominant follicle. Cows were ovariectomized
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 24/02/2012
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10. Association of recombinant bovine somatotropin (rBST) with equine chorionic gonadotropin (eCG) on antral follicle count and oocyte production in Holstein and Tabapuã heifers
The objective of this study was to investigate whether the use of rbST and eCG prior to ultrasound-guided follicular aspiration (OPU) improves oocyte yield and quality in Tabapuã and Holstein heifers. The study was conducted in two phases, 20 days apart, in a change-over design. The dominant follicle was ablated two days (D-2) before two treatments: stimula
R. Bras. Zootec.. Publicado em: 2012-12
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11. Avaliação do swim-up e do gradiente descontinuo de densidade na pré-seleção do sexo de espermatozoides na produção de embriões bovinos
The purpose of this work was to associate the modified swim-up method with centrifugation in density gradient for the separation of X-bearing spermatozoa. Sperm viability and integrity were evaluated through the Trypan Blue/Giemsa staining method. Quality control of centrifuged spermatozoa was performed in in vitro produced embryos. The results were validate
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Publicado em: 2012-06
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12. Use of chromatin modifying agents in bovine somatic cell nuclear transfer / Uso de agentes modificadores de cromatina na transferência nuclear de células somáticas em bovinos
Embora a transferência nuclear de células somáticas (TNCS) seja uma ferramenta promissora, seu amplo uso é impedido devido às altas taxas de mortalidade durante o desenvolvimento dos animais clonados. Acredita-se que a reprogramação epigenética anormal seja a principal causa desta baixa eficiência. Nós hipotetizamos que agentes modificadores de cro
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 13/12/2011