Botryodiplodia Theobromae
Mostrando 13-20 de 20 artigos, teses e dissertações.
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13. Presence of a major (storage?) protein in dormant spores of the fungus Botryodiplodia theobromae.
Approximately 23% of the protein isolated from dormant spores of Botryodiplodia theobromae consisted of a single polypeptide; the polypeptide is probably degraded during germination.
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14. Biosynthesis and degradation of storage protein in spores of the fungus Botryodiplodia theobromae.
Muiridin, a spore-specific protein of the fungus Botryodiplodia theobromae, comprises about 25% of the mature pycnidiospore protein. It has an apparent molecular weight of 16,000 to 17,000 and is rich in glutamine, asparagine, and arginine. Muiridin is synthesized in developing spores via a precursor with an apparent molecular weight of 24,000. Two other pol
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15. Presence of Polyribosomes in Conidiospores of Botryodiplodia theobromae Harvested with Nonaqueous Solvents1
Polyribosomes detected in extracts of spores harvested with water also were found in extracts prepared from spores harvested with nonaqueous fluids.
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16. Protein Synthesis During Fungal Spore Germination II. Aminoacyl-soluble Ribonucleic Acid Synthetase Activities During Germination of Botryodiplodia theobromae Spores1
The specific activities of 13 aminoacyl-soluble ribonucleic acid (sRNA) synthetases were measured at various time intervals during the germination of Botryodiplodia theobromae conidiospores. The enzyme activities were low or absent in ungerminated spores, and they increased rapidly as germination proceeded. When extracts of the ungerminated spores were prepa
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17. Protein Synthesis During Fungal Spore Germination IV. Transfer Ribonucleic Acid from Germinated and Ungerminated Spores1
Transfer ribonucleic acid (tRNA) fractions isolated from germinated and ungerminated spores of Botryodiplodia theobromae and Rhizopus stolonifer had acceptor activity for all 20 amino acids commonly found in protein, when tested with an enzyme fraction from germinated spores. Accordingly, it is unlikely that the absence of tRNA for a particular amino acid li
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18. In Vitro Translation of Polyadenylate-Containing RNAs from Dormant and Germinating Spores of the Fungus Botryodiplodia theobromae
Polyadenylated RNA isolated by oligodeoxythymidylate-cellulose chromatography from spores of the fungus Botryodiplodia theobromae was translated in a cell-free protein-synthesizing system derived from wheat embryo. Reaction conditions which would yield efficient and accurate in vitro translation of the spore RNA were established. Dual isotopically labeled mi
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19. Mitochondrial biogenesis during fungal spore germination: effects of the antilipogenic antibiotic cerulenin upon Botryodiplodia spores.
Germination of spores of the fungus Botryodiplodia theobromae was inhibited by the antilipogenic antibiotic cerulenin. The spores remained viable in the presence of the antibiotic, however, and after prolonged incubation they were able to overcome the inhibition. Cerulenin inhibition of germination was reversed by Tween 40 and Tween 60 (derivatives of palmit
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20. Mitochondrial biogenesis during fungal spore germination: respiratory cytochromes of dormant and germinating spores of Botryodiplodia.
The mitochondrial respiratory cytochrome contents of dormant and germinating conidia of Botryodiplodia theobromae were examined. Oxidized versus reduced difference spectra at 77 degrees K of whole mitochondria from physiologically mature germinated spores showed a typical a-band pattern for cytochromes c, b, and a, with absorption maxima at 549, 554 + 559, a