Boophilus Microplus Cystein Endopeptidase
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1. A Boophilus microplus vitellin-degrading cysteine endopeptidase
Here we describe the purification and characterization of a vitellin (VT) degrading cysteine endopeptidase (VTDCE) from eggs of the hard tick Boophilus microplus. A homogeneous enzyme preparation was obtained by chromatographic fractionation on ion-exchange and gel filtration columns and an autolysis step. This step consisted of incubation of a semipurified
Publicado em: 2011