Bluetongue
Mostrando 13-24 de 107 artigos, teses e dissertações.
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13. Bluetongue virus: comparative evaluation of enzyme-linked immunosorbent assay, immunodiffusion, and serum neutralization for detection of viral antibodies.
Comparative studies on the detection of bovine serum immunoglobulin G antibodies to bluetongue virus with an enzyme-linked immunosorbent assay, an immunodiffusion method, and a serum neutralization assay demonstrated complete concordance between the enzyme-linked immunosorbent assay and the serum neutralization assay results. However, the immunodiffusion met
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14. Soluble Antigen of Blue-Tongue Virus
A soluble viral antigen with possible diagnostic utility was obtained from blue-tongue virus-infected lamb kidney cells. It is a noninfectious proteinaceous substance without nucleic acid. The antigen is immunologically identical to one component of the viral particle, and in agar-gel diffusion tests it reacted with antiserum against several blue-tongue viru
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15. Studies on the Topography of Reovirus and Bluetongue Virus Capsid Proteins
Protein labeling experiments confirm the surface location of proteins 2 and 5 in bluetongue virus, and proteins σ3 and μ2 in reovirus. Lambda 2 is the major surface component of the reovirus core, and proteins 1, 3, and 4 appear to be the outer components of the bluetongue virus subviral particle.
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16. Simple procedure for preparation of bluetongue virus and epizootic hemorrhagic disease virus antigens for agar gel immunodiffusion.
A simplified procedure was developed for preparing soluble antigen from two related orbiviruses, bluetongue and epizootic hemorrhagic disease viruses, for agar gel immunodiffusion. The antigens gave excellent results in both micro-agar gel diffusion (agar gel precipitin) and macro-agar gel diffusion (bluetongue immunodiffusion). Minor modification in the spa
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17. Comparison of some storage and isolation methods to recover bluetongue virus from bovine blood.
Bovine blood containing bluetongue virus was stored as whole blood with EDTA (4 degrees C), as washed cellular components (4 degrees C), and as washed cellular components with 10% DMSO (-70 degrees C). Periodic isolation attempts were made over a period of 330 days in four cell lines and embryonating chicken eggs (intravenous inoculation). Bluetongue virus w
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18. Bluetongue virus, an exceptionally potent interferon inducer in mice.
The attenuated American BT-8 strain of bluetongue virus is 5 to 10 times more potent an interferon inducer than any other viral or nonviral agent reported to date, including as much as 600,000 units/ml of plasma by 8 h after intravenous injection.
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19. Bluetongue virus type 17 can exist in a latent state in MDBK cells.
An infection with bluetongue virus type 17 can be regulated by the temperature of incubation to be either persistent, producing low levels of virus; lytic, producing a high titer of released virus; or latent, producing no detectable virus. The persistent and latent states are reversible.
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20. The Core of Bluetongue Virus Binds Double-Stranded RNA
Double-stranded RNA (dsRNA) viruses conceal their genome from the host to avoid triggering unfavorable cellular responses. The crystal structure of the core of one such virus, bluetongue virus, reveals an outer surface festooned with dsRNA. This may represent a deliberate strategy to sequester dsRNA released from damaged particles to prevent host cell shutof
American Society for Microbiology.
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21. Association of bluetongue virus gene segment 5 with neuroinvasiveness.
Two strains (UC-2 and UC-8) of bluetongue virus were used to determine genetic factors influencing neuroinvasiveness. Reassortants were produced in vitro, and the parental origins of their genes were determined by polyacrylamide gel electrophoresis profiles and restriction endonuclease digestion. Gene segment 5 of UC-8 correlated with neuroinvasiveness of re
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22. Detection of bluetongue virus by using bovine endothelial cells and embryonated chicken eggs.
Two systems, inoculation of bovine endothelial cells and of embryonated chicken eggs, were compared for detection of bluetongue virus (BTV) in blood specimens from experimentally inoculated sheep. For all BTV serotypes tested, embryonated chicken eggs detected longer periods of viremia than did bovine endothelial cells, primarily by detecting BTV in samples
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23. Assembly of double-shelled, viruslike particles of bluetongue virus by the simultaneous expression of four structural proteins.
Bluetongue is a disease of ruminants. The etiologic agent is bluetongue virus (BTV), a gnat-transmitted member of the Orbivirus genus of the Reoviridae. The virus has a genome of 10 double-stranded RNA species L1 to L3, M4 to M6, S7 to S10). The L2 and M5 genes of BTV which encode the outer capsid proteins VP2 and VP5, respectively, were inserted into a reco
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24. In vitro phosphorylation and purification of a nonstructural protein of bluetongue virus with affinity for single-stranded RNA.
A phosphorylated, nonstructural protein of bluetongue virus, NS2, is synthesized throughout the replication cycle in comparatively large amounts. The protein was detected in both the soluble and particulate fraction of the cytoplasm of infected cells. The particulate NS2 could be solubilized in 0.5 M NaCl. It was found that NS2 in the particulate fraction an