Blastocladiella Emersonii
Mostrando 13-24 de 44 artigos, teses e dissertações.
-
13. Esterase activity during the life cycle of Blastocladiella emersonii.
Total esterase activity was measured in extracts on Blastocladiella throughout its life cycle by the degradation of alpha-naphthyl acetate. A fivefold incease in activity, apparently due to the synthesis of new enzymes, was found during sporulation.
-
14. Alkaline phosphatase of Blastocladiella emersonii: partial purification and characterization.
Alkaline phosphomonoesterase (EC 3.1.3.1) activity from Blastocladiella emersonii, while displaying typically broad substrate specificity for phosphorylated organic compounds, exhibited nearly complete substrate preference for N-acetylglucosamine-6-phosphate over N-acetylglucosamine-1-phosphate. Enzyme in zoospore extracts was purified 43-fold by differentia
-
15. Cyclic nucleotide-independent protein kinases from ribosomes and phosphorylation of a single 40S ribosomal subunit protein in zoospores of Blastocladiella emersonii.
Cyclic nucleotide-independent protein kinase (EC 2.7.1.37) activity was found in the nuclear cap organelle, within which ribosomes of zoospores of Blastocladiella emersonii are sequestered. Two protein kinase activities were resolved from the high-salt wash fraction of zoospore ribosomes by selective adsorption to DEAE-cellulose. Both enzymes phosphorylated
-
16. FURTHER EVIDENCE FOR THE ROLE OF THE TRICARBOXYLIC ACID CYCLE IN MORPHOGENESIS IN BLASTOCLADIELLA EMERSONII1
-
17. Growth and differentiation of the water mold Blastocladiella emersonii: cytodifferentiation and the role of ribonucleic acid and protein synthesis.
-
18. Cytochrome Oxidase Activity in Blastocladiella emersonii1
Studies of cytochrome oxidase in isolated mitochondria of Blastocladiella emersonii Cant. and Hyatt show that the enzyme was present in zoospores and throughout the development of ordinary colorless sporangia and of resistant sporangia. The enzyme activity was present in KCl, NaCl, NH4Cl, and KHCO3 induced resistant sporangia, and was shown to be as active o
-
19. Cyclic nucleotide metabolism coupled to cytodifferentiation of Blastocladiella emersonii.
Cyclic GMP and cyclic AMP levels during growth and differentiation of B. emersonii were measured by use of chemical, biochemical, and immunologic assays. Of particular interest was the finding that net synthesis of cyclic GMP occurred during a single stage of its life cycle, sporulation, when intracellular levels increased 50- to 100-fold in a process requir
-
20. Specific Inhibitors of the Three RNA Polymerases from the Aquatic Fungus Blastocladiella emersonii
Specific inhibitors of each of the three RNA polymerases of Blastocladiella emersonii have been found. Cycloheximide specifically inhibited the in vitro activity of the DEAE-fraction I enzyme, alpha-amanitin specifically inhibited the DEAE-fraction II enzyme, and rifampicin specifically inhibited the fraction III enzyme. DNA stimulation and dependency on the
-
21. Structure, Expression, and Functional Analysis of the Gene Coding for Calmodulin in the Chytridiomycete Blastocladiella emersonii
The single calmodulin (CaM) gene and the corresponding cDNA of the chytridiomycete Blastocladiella emersonii were isolated and characterized. The CaM gene is interrupted by three introns and transcribed in a single 0.7-kb mRNA species encoding a predicted protein 91% identical to human CaM. B. emersonii CaM has been expressed in Escherichia coli as a fusion
American Society for Microbiology.
-
22. Endogenous electrical currents in the water mold Blastocladiella emersonii during growth and sporulation.
We have explored the pattern of electrical currents generated by single cells of the water mold Blastocladiella emersonii at several stages of its life cycle. Extracellular currents were measured with a vibrating probe constructed after the design of Jaffe and Nuccitelli [Jaffe, L. F. & Nuccitelli, R. (1974) J. Cell Biol. 63, 614-628]. In growing cells posit
-
23. Isolation, Characterization, and Expression of the Gene Encoding the β Subunit of the Mitochondrial Processing Peptidase from Blastocladiella emersonii
A 2.3-kb BamHI-KpnI fragment was isolated from a partial genomic library and shown by nucleotide sequence analysis to contain the entire coding region of the gene encoding the β subunit of the Blastocladiella mitochondrial processing peptidase (β-MPP). The predicted β-MPP protein has 465 amino acids and a calculated molecular mass of 50.8 kDa. S1 nuclease
American Society for Microbiology.
-
24. Lipid Composition of the Zoospores of Blastocladiella emersonii1
The zoospores of Blastocladiella emersonii, when derived from cultures grown on solid media, contain about 11% total lipid. This lipid was separated chromatographically on silicic acid into neutral lipid (46.6%), glycolipid (15.8%), and phospholipid (37.6%). Each class was fractionated further on columns of silicic acid, Florisil, or diethylaminoethyl-cellul