Bicyclomycin
Mostrando 1-10 de 10 artigos, teses e dissertações.
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1. Florfenicol e biciclomicina: eficÃcia in vitro contra bactÃrias patogÃnicas de peixes de Ãgua doce. / In vitro efficacy of florfenicol e bicyclomycin to pathogenic bacteria from freshwater fish.
The Streptococcus agalactiae and the motile aeromonads are major pathogens for several tropical fish species, causing encephalitis and septicemia outbreaks, respectively, with high mortality in intensive culture systems. The use of antibiotic is the main control measure. The objective of this work was to determine the minimum inhibitory concentration (MIC) o
Publicado em: 2006
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2. Transcription termination factor Rho is essential for Micrococcus luteus.
The growth of Micrococcus luteus, a soil microorganism that belongs to the high-G+C gram-positive phylogenetic group, is prevented by bicyclomycin, an antibiotic that inhibits the activity of the M. luteus transcription termination factor Rho. A mutant that can grow in 0.3 mM bicyclomycin has a Rho that is insensitive to bicyclomycin and has the single amino
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3. Bicyclomycin sensitivity and resistance affect Rho factor-mediated transcription termination in the tna operon of Escherichia coli.
The growth-inhibiting drug bicyclomycin, known to be an inhibitor of Rho factor activity in Escherichia coli, was shown to increase basal level expression of the tryptophanase (tna) operon and to allow growth of a tryptophan auxotroph on indole. The drug also relieved polarity in the trp operon and permitted growth of a trp double nonsense mutant on indole.
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4. Morphological Changes of Escherichia coli Induced by Bicyclomycin
Electron microscopic studies with Escherichia coli revealed that bicyclomycin inhibits septum formation and converts the cells to filamentous forms. The antibiotic induced high undulation and numerous blebs of the outer membrane. Sometimes cytoplasmic contents leaked into the lumen of the bleb through a disrupted region of the membrane. Breakage of the outer
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5. Effects of Bicyclomycin on RNA- and ATP-Binding Activities of Transcription Termination Factor Rho
Bicyclomycin is a commercially important antibiotic that has been shown to be effective against many gram-negative bacteria. Genetic and biochemical evidence indicates that the antibiotic interferes with RNA metabolism in Escherichia coli by inhibiting the activity of transcription termination factor Rho. However, the precise mechanism of inhibition is not c
American Society for Microbiology.
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6. rho Is Not Essential for Viability or Virulence in Staphylococcus aureus
We have identified the gene for transcription termination factor Rho in Staphylococcus aureus. Deletion of rho in S. aureus reveals that it is not essential for viability or virulence. We also searched the available bacterial genomic sequences for homologs of Rho and found that it is broadly distributed and highly conserved. Exceptions include Streptococcus
American Society for Microbiology.
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7. In Vitro Activity of 39 Antimicrobial Agents Against Treponema hyodysenteriae
The in vitro activities of 39 antimicrobial agents against 23 isolates of Treponema hyodysenteriae, the majority of which were field isolates, were determined by the agar dilution technique. Quinoxalines, pleuromutilin, nitroimidazoles, and nitrofuran were the most active. Their activities ranged from ≤0.10 to 1.56 μg/ml. Lincomycin, penicillins, chloramp
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8. Characterization of Mutations Contributing to Sulfathiazole Resistance in Escherichia coli
A sulfathiazole-resistant dihydropteroate synthase (DHPS) present in two different laboratory strains of Escherichia coli repeatedly selected for sulfathiazole resistance was mapped to folP by P1 transduction. The folP mutation in each of the strains was shown to be identical by nucleotide sequence analysis. A single C→T transition resulted in a Pro→Ser
American Society for Microbiology.
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9. Rho-Dependent Transcription Termination in the tna Operon of Escherichia coli: Roles of the boxA Sequence and the rut Site
Expression of the tryptophanase (tna) operon of Escherichia coli is regulated by catabolite repression and by tryptophan-induced transcription antitermination. Tryptophan induction prevents Rho-dependent transcription termination in the leader region of the operon. Induction requires translation of a 24-residue leader peptide-coding region, tnaC, containing
American Society for Microbiology.
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10. Loss of overproduction of polypeptide release factor 3 influences expression of the tryptophanase operon of Escherichia coli.
Expression of the tryptophanase (tna) operon of Escherichia coli is regulated by catabolite repression and by tryptophan-induced inhibition of Rho-mediated transcription termination. Previous studies indicated that tryptophan induction might involve leader peptide inhibition of ribosome release at the stop codon of tnaC, the coding region for the operon-spec