Balb C 3t3 A31 Cells
Mostrando 1-12 de 20 artigos, teses e dissertações.
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1. Emprego da citotoxicidade basal in vitro na redução do número de animais em ensaios de avaliação da toxicidade oral aguda: a grandisina e seu metabólito majoritário como protótipos / Use of basal cytotoxicity in vitro in reducing the number of animals tests in the evaluation of acute oral toxicity: a grandisin and its major metabolite as prototypes
A substituição ou redução do uso de animais em experimentos para a avaliação de toxicidade tem sido bastante encorajada e tem recebido grandes incentivos, inclusive financeiros, governamentais e institucionais. No entanto, a substituição completa da maioria dos testes mandatórios por agências reguladoras do setor ainda não é realidade, a exemplo,
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 14/04/2009
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2. Emprego da citotoxicidade basal in vitro na redução do número de animais em ensaios de avaliação da toxicidade oral aguda: a grandisina e seu metabólito majoritário como protótipos / Use of basal cytotoxicity in vitro in reducing the number of animals tests in the evaluation of acute oral toxicity: a grandisin and its major metabolite as prototypes
A substituição ou redução do uso de animais em experimentos para a avaliação de toxicidade tem sido bastante encorajada e tem recebido grandes incentivos, inclusive financeiros, governamentais e institucionais. No entanto, a substituição completa da maioria dos testes mandatórios por agências reguladoras do setor ainda não é realidade, a exemplo,
Publicado em: 2009
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3. Envolvimento de células ER-MP58+ na produção de IL-12 em linfonodos drenantes da infecção inicial por Leishmania major em camundongos BALB/c
The development of a Th1 immune response is the key event to prevent Leishmania infection and is linked with the IL-12 production by monocytes dendritic cells macrophages and neutrophils The IL-12 signal induces the increase of IFN- production by T cells favoring the profile of the Th1 immune response and the resistance phenotype to the infection In the vert
Publicado em: 2006
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4. Transformation of mouse BALB/c 3T3 cells with human basic fibroblast growth factor cDNA.
The expression of human basic fibroblast growth factor (bFGF) cDNA in mouse BALB/c 3T3 clone A31 cells induced morphological transformation. These transformed cells grew well and reached more than a sixfold-higher saturation density than parental A31 cells even in serum-free medium. They were able to form colonies in soft agar. The phenotypic alteration in t
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5. Host range studies of FLOPC-1 murine myeloma C particles.
The host range of the C particle produced by FLOPC-1 myeloma cells, FLOPC-1 murine myeloma-associated virus (FL-MuMAV), was assessed in terms of its ability to productively infect and/or induce new viral antigens in a variety of different cell lines. Production of C particle-like structures by cells exposed to FL-MuMAV) was determined by incorporation of [3H
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6. Characterization of a New Murine Cellular DNA Polymerase
A new DNA polymerase (peak A) has been identified in the high-speed pellet fraction of two subclones of nonvirus producing Balb/3T3 cells. The activity is associated with a molecule of approximately 70,000 molecular weight and chromatographs in two systems like the mouse type-C viral reverse transcriptase and a similar enzyme from the high-speed pellet fract
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7. Ras proteins are essential and selective for the action of insulin-like growth factor 1 late in the G1 phase of the cell cycle in BALB/c murine fibroblasts.
BALB/c 3T3 cells (A31 cells) require the sequential action of growth factors in order to proliferate from a quiescent growth state. Insulin-like growth factor I (IGF-I) is needed late in the G1 phase of the cell cycle, a time at which expression of the c-Ha-ras protooncogene is near maximal. An anti-ras antibody, introduced by microinjection, specifically bl
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8. Biochemical Studies on Bovine Adenovirus Type 3 IV. Transformation by Viral DNA and DNA Fragments
By the calcium technique, intact DNA of bovine adenovirus type 3 (BAV3) was found to transform A31 cells, a clone of BALB/3T3. Transforming activity was resistant to RNase and Pronase but sensitive to DNase. The efficiency of transformation was approximately 5 to 10 foci per μg of DNA. Attempts were also made to test for transforming activity of BAV3 DNA fr
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9. Transformed and nontransformed cells differ in stability and cell cycle regulation of a binding activity to the murine thymidine kinase promoter.
A DNA binding activity to an upstream region of the murine thymidine kinase gene is regulated differently in a transformed and nontransformed cell line pair. Differences in regulation were observed (i) after serum levels were reduced, (ii) when serum levels were returned to initial high levels, and (iii) while protein synthesis was inhibited. After reduction
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10. Shared idiotopes among monoclonal antibodies specific for A/PR/8/34 (H1N1) and X-31(H3N2) influenza viruses.
A monoclonal antibody specific for the hemagglutinin (HA) of influenza A X-31 (H3N2) virus (X-31) was obtained during a fusion of spleen cells from a BALB/c mouse immunized with influenza A/PR/8/34 (H1N1) virus (PR8). This monoclonal antibody (Py206) shares crossreactive idiotopes expressed on several monoclonal antibodies specific for PR8 HA and X-31 HA as
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11. FGF3 from Xenopus laevis.
Fibroblast growth factor 3 (FGF3) was first identified as the product of a cellular oncogene activated by mouse mammary tumour virus but its normal role appears to be in the developing embryo. To gain further insights into its function, we have isolated sequences encoding the FGF3 homologue in Xenopus laevis, XFGF3. COS-1 cells transfected with XFGF3 cDNA ex
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12. Induction of c-fos and c-myc mRNA by epidermal growth factor or calcium ionophore is cAMP dependent.
Phorbol esters activate protein kinase C and induce expression of the c-fos and c-myc protooncogenes in density-arrested BALB/c 3T3 (A31) cells; in contrast, epidermal growth factor (EGF) does not activate protein kinase C and is a poor inducer of c-fos and c-myc in these confluent cells. We show that, when A31 cells were subconfluent and made quiescent by s