Bacterial Typing Techniques
Mostrando 1-12 de 23 artigos, teses e dissertações.
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1. Typing of Pseudomonas aeruginosa from hospitalized patients : a comparison of susceptibility and biochemical profiles with genotype
Typing techniques are essential for understanding hospital epidemiology, permitting the elucidation of the source of infection and routes of bacterial transmission. Although DNA-based techniques are the “gold standard” for the epidemiological study of Pseudomonas aeruginosa, antibiotic profiles and biochemical results are used because they are easy to pe
Publicado em: 2010
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2. Epidemiological characterization of resistance and PCR typing of shigella flexneri and shigella sonnei strains isolated from bacillary dysentery cases in Southeast Brazil / Caracterização biologica e molecular de amostras de shigella flexneri e shigella sonnei isoladas da regiao de Campinas-SP
Shigella spp are gram-negative, anaerobic facultative, non-motile, and non-sporulated bacilli of the Enterobacteriaceae family, responsible for Shigellosis or the Bacillary Dysentery (BD) disease, an important cause of worldwide morbidity and mortality. The pathogenic determinants of Shigella spp include high molecular weight plasmids responsible for the bac
Publicado em: 2007
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3. Avaliação da influência do tratamento diretamente observado (DOT) com e sem profilaxia na transmissão da tuberculose em centros médicos de saúde do município do Rio de Janeiro, utilizando técnicas de tipagem molecular em cepas de M. tuberculosis / Evaluation influence of the treatment directly observed (DOT) with and without Prophylaxis in the transmission of the tuberculosis in medical centers of health of the city of Rio De Janeiro, using tuberculosis techniques of molecular typing in cepas of M. tuberculosis
Tuberculosis (TB) is a major cause of morbidity and mortality worldwide, particularly in developing countries. In the city of Rio de Janeiro, 7,000 new TB cases are reported annually, for an incidence de 105 per 100,000 inhabitants. In three Community Health Centers (CHCs) of Planning Area 1 (AP-1.0) of Rio de Janeiro, the section of the city with the highes
Publicado em: 2006
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4. Typing of Pseudomonas aeruginosa from hospitalized patients: a comparison of susceptibility and biochemical profiles with genotype
Typing techniques are essential for understanding hospital epidemiology, permitting the elucidation of the source of infection and routes of bacterial transmission. Although DNA-based techniques are the "gold standard" for the epidemiological study of Pseudomonas aeruginosa, antibiotic profiles and biochemical results are used because they are easy to perfor
Brazilian Journal of Medical and Biological Research. Publicado em: 2004-01
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5. Analise do polimorfismo genetico de cepas de Mycobacterium tuberculosis isoladas de pacientes portadores de tuberculose pulmonar atendidos no Hospital das Clinicas da UNICAMP
Tuberculosis is a major concem in developing countries. The contribution of genotyping techniques to trace epidemiological chains is remarkable, IS 6110RFLP being the preferred typing method. AIDS epidemics has had a major impact on the incidence of tuberculosis. Not so many genotyping studies have been made in Brazil in order to check for the number of IS61
Publicado em: 2003
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6. Application of Pulsed-Field Gel Electrophoresis and Binary Typing as Tools in Veterinary Clinical Microbiology and Molecular Epidemiologic Analysis of Bovine and Human Staphylococcus aureus Isolates
Thirty-eight bovine mammary Staphylococcus aureus isolates from diverse clinical, temporal, and geographical origins were genotyped by pulsed-field gel electrophoresis (PFGE) after SmaI digestion of prokaryotic DNA and by means of binary typing using 15 strain-specific DNA probes. Seven pulsed-field types and four subtypes were identified, as were 16 binary
American Society for Microbiology.
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7. Multilocus Sequence Typing Is a Reliable Alternative Method to DNA Fingerprinting for Discriminating among Strains of Candida albicans
Multilocus sequence typing (MLST) has emerged as a powerful new DNA-typing tool for the evaluation of intraspecies genetic relatedness. This method relies on DNA sequence analysis of nucleotide polymorphisms in housekeeping genes and has shown a high degree of intraspecies discriminatory power for bacterial and fungal pathogens. However, the results of the M
American Society for Microbiology.
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8. Analysis of clonal relationships among isolates of Shigella sonnei by different molecular typing methods.
Shigella sonnei is a major cause of diarrheal disease in developed as well as in developing countries. Epidemiologic studies of this organism have been limited by the lack of a simple and effective method for comparing strains. In this study, we have compared different molecular typing methods, i.e., plasmid profile analysis, restriction endonuclease analysi
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9. Use of PCR to study epidemiology of Serratia marcescens isolates in nosocomial infection.
A method to characterize strains of Serratia marcescens based on the PCR amplification of enterobacterial repetitive intergenic consensus sequences has been developed. The PCR fingerprints were generated from boiled supernatants prepared directly from bacterial colonies without the need for DNA extraction. The technique was applied to isolates obtained durin
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10. Risk Factors for Colonization and Infection in a Hospital Outbreak Caused by a Strain of Klebsiella pneumoniae with Reduced Susceptibility to Expanded-Spectrum Cephalosporins
Between February 2001 and January 2002, an increase in the number of Klebsiella pneumoniae isolates with reduced susceptibility to expanded-spectrum cephalosporins (RSKp) was detected in the neonatal unit of the Juan Canalejo Hospital, and 21 patients were either colonized or infected by the bacterial isolates. The current “gold standard” method for typi
American Society for Microbiology.
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11. Ribotype diversity of Listeria monocytogenes strains associated with outbreaks of listeriosis in ruminants.
Ribotyping is a molecular method for the characterization, identification, and typing of bacterial isolates that has value in epidemiological studies. To demonstrate the utility of this technique for typing of Listeria monocytogenes, four outbreaks of epizootic listeriosis in ruminants were investigated through coordinated detection and characterization meth
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12. eBURST: Inferring Patterns of Evolutionary Descent among Clusters of Related Bacterial Genotypes from Multilocus Sequence Typing Data
The introduction of multilocus sequence typing (MLST) for the precise characterization of isolates of bacterial pathogens has had a marked impact on both routine epidemiological surveillance and microbial population biology. In both fields, a key prerequisite for exploiting this resource is the ability to discern the relatedness and patterns of evolutionary
American Society for Microbiology.