Automatic Sequencing
Mostrando 13-24 de 43 artigos, teses e dissertações.
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13. Associação da infecção pelo vírus da hepatite c e pelo vírus da imunodeficiência humana em moçambicanos e brasileiros
The prevalence of HIV/HCV co-infection has been reported from Brazil and from some Sub Saharan African countries; however, no data is available from Mozambique. This study aimed at reporting, the prevalence of the co-infection and describe the profile of the selected patients from an Infectious Diseases Unit in Maputo, Mozambique and to compare their profile
Publicado em: 2007
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14. Moniliophthora perniciosa genome: assembly and annotation of mitochondrion and development of a semi-automatic system of genes annotation / Genoma de Moniliophthora perniciosa : montagem e anotação da mitocondria e desenvolvimento de sistema de anotação semi-automatico de genes
The mitochondrial genome (mtDNA) of the fungus Moniliophthora perniciosa was completely sequenced and it contains 109103 bases pair, with 31% of bases GC, smaller percentage than found in the sequences of the nuclear genome (47%). It is the largest mitochondrial genome of fungus described to the moment, and its size is consequence of great intergenic space,
Publicado em: 2006
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15. Estudo de mutações em genes responsaveis por diferentes formas de disturbios do desenvolvimento cortical
Cortical development malformations (CDM) are one of the most important causes of epilepsy and developmental delay. Extensive molecular genetic studies have resulted in gene discovery for CDM such as periventricular nodular heterotopia (PNH), lisencephaly/ subcortical band heterotopia spectrum (LIS/SBH) and schizencephaly. The main goals of this project were
Publicado em: 2003
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16. Exoquence DNA sequencing.
We have developed a strategy for DNA sequencing based on exonuclease III digestion followed by double strand specific endonuclease digestion and direct dideoxynucleotide sequencing reaction. This strategy eliminates the need for subcloning, oligonucleotide primers, and prior knowledge of the DNA to be sequenced. All template and primer duplexes needed for se
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17. Capillary gel electrophoresis for rapid, high resolution DNA sequencing.
Capillary gel electrophoresis has been demonstrated for the separation and detection of DNA sequencing samples. Enzymatic dideoxy nucleotide chain termination was employed, using fluorescently tagged oligonucleotide primers and laser based on-column detection (limit of detection is 6,000 molecules per peak). Capillary gel separations were shown to be three t
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18. Direct PCR sequencing with boronated nucleotides.
A method is described to simultaneously amplify and sequence DNA using a new class of nucleotides containing boron. During the polymerase chain reaction, boron-modified nucleotides, i.e. 2'-deoxynucleoside 5'-alpha-[P-borano]-triphosphates, are incorporated into the product DNA. The boranophosphate linkages are resistant to nucleases and thus the positions o
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19. Sequencing of oligonucleotide phosphorothioates based on solid-supported desulfurization.
We described a solid-supported desulfurization procedure allowing easy access to the sequence analysis of oligonucleotide phosphorothioates. The described method is based upon selective removal of the 2-cyanoethyl phosphate protecting groups, followed by iodine-promoted desulfurization of the resulting phosphorothioate diesters. Automatic oxidation of oligon
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20. Automatic reading of DNA sequencing gel autoradiographs using a large format digital scanner.
We have developed a large format digital scanner for several applications in nucleic acid analysis. Here we describe the scanning of autoradiographs of DNA sequencing gels and a set of programs for reading the base sequence. The programs correct distortions in the gel, recognize bands by their characteristic shape and assign bases to bands by weighting band
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21. The application of numerical estimates of base calling accuracy to DNA sequencing projects.
During DNA sequencing projects one of the most labour intensive and highly skilled tasks is to view the original trace descriptions of gels and to adjudicate between conflicting readings. Given the current methods of calculating a consensus, the majority of the time employed in viewing traces and editing readings is actually devoted to making the poorer data
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22. Projector: automatic contig mapping for gap closure purposes
Projector was designed for automatic positioning of contigs from an unfinished prokaryotic genome onto a template genome of a closely related strain or species. Projector mapped 84 contigs of Lactococcus lactis MG1363 (corresponding to 81% of the assembly nucleotides) against the genome of L.lactis IL1403. Ninety three percent of subsequent gap closure PCRs
Oxford University Press.
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23. A semi-automated method for the reading of nucleic acid sequencing gels.
A collection of computer programs is described which permit automatic entering of nucleotide sequence data directly from an autoradiograph into a computer. This collection, called DIGITPAD, makes use of a digitizing tablet for the data entry and allows the rapid and accurate transfer of the sequence into the computer.
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24. Tools and resources for identifying protein families, domains and motifs
With the large influx of raw sequence data from genome sequencing projects, there is a need for reliable automatic methods for protein sequence analysis and classification. The most useful tools use various methods for identifying motifs or domains found in previously characterized protein families. This article reviews the tools and resources available on t
BioMed Central.