Atp Diphosphohydrolase
Mostrando 13-23 de 23 artigos, teses e dissertações.
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13. Cross-immunoreactivity between anti-potato apyrase antibodies and mammalian ATP diphosphohydrolases: potential use of the vegetal protein in experimental schistosomiasis
We have previously showed that Schistosoma mansoni ATP-diphosphohydrolase and Solanum tuberosum potato apyrase share epitopes and the vegetable protein has immunostimulatory properties. Here, it was verified the in situ cross-immunoreactivity between mice NTPDases and anti-potato apyrase antibodies produced in rabbits, using confocal microscopy. Liver sample
Memórias do Instituto Oswaldo Cruz. Publicado em: 2006-10
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14. Extracellular ATP and ADP hydrolyzed by the NTPDase1 enzyme in lymphocytes from B-chronic lymphocytic leukemia patients / Hidrólise extracelular de ATP e ADP pela enzima NTPDase1 em linfócitos de pacientes com leucemia linfocítica crônica-B
The NTPDase1 enzymatic activity (E.C.3.6.1.5, Apyrase, ecto-ATP-diphosphohydrolase, CD39) which hydrolyses ATP and ADP, was analyzed in peripheral lymphocytes from patients with B chronic lymphocytic leukemia (B-CLL) which the main characteristic is the accumulation of monoclonal mature B-lymphocyte. The sample was composed by 23 patients with B-CLL whose we
Publicado em: 2006
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15. Ectonucleotidase activities in Sertoli cells from immature rats
Sertoli cells have been shown to be targets for extracellular purines such as ATP and adenosine. These purines evoke responses in Sertoli cells through two subtypes of purinoreceptors, P2Y2 and P A1. The signals to purinoreceptors are usually terminated by the action of ectonucleotidases. To demonstrate these enzymatic activities, we cultured rat Sertoli cel
Brazilian Journal of Medical and Biological Research. Publicado em: 2001-10
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16. Brain ischemia alters platelet ATP diphosphohydrolase and 5'-nucleotidase activities in naive and preconditioned rats
The effects of transient forebrain ischemia, reperfusion and ischemic preconditioning on rat blood platelet ATP diphosphohydrolase and 5'-nucleotidase activities were evaluated. Adult Wistar rats were submitted to 2 or 10 min of single ischemic episodes, or to 10 min of ischemia 1 day after a 2-min ischemic episode (ischemic preconditioning) by the four-vess
Brazilian Journal of Medical and Biological Research. Publicado em: 2000-11
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17. Studies on ATP-diphosphohydrolase nucleotide-binding sites by intrinsic fluorescence
Potato apyrase, a soluble ATP-diphosphohydrolase, was purified to homogeneity from several clonal varieties of Solanum tuberosum. Depending on the source of the enzyme, differences in kinetic and physicochemical properties have been described, which cannot be explained by the amino acid residues present in the active site. In order to understand the differen
Brazilian Journal of Medical and Biological Research. Publicado em: 2000-07
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18. Intestinal alkaline phosphatase regulates protective surface microclimate pH in rat duodenum
Regulation of localized extracellular pH (pHo) maintains normal organ function. An alkaline microclimate overlying the duodenal enterocyte brush border protects the mucosa from luminal acid. We hypothesized that intestinal alkaline phosphatase (IAP) regulates pHo due to pH-sensitive ATP hydrolysis as part of an ecto-purinergic pH regulatory system, comprised
Blackwell Science Inc.
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19. A Ca++-Dependent and -Selective Ionophore as Part of the Ca++ + Mg++-Dependent Adenosinetriphosphatase of Sarcoplasmic Reticulum
Solubilized Ca++ + Mg++-dependent adenosinetriphosphatase (EC 3.6.1.3; ATP diphosphohydrolase) from sarcoplasmic reticulum increased bimolecular lipid membrane (oxidized cholesterol) conductance several hundred-fold. The relative conductance change and the relative permeability elicited by this material has the following sequence: Ba++ > Ca++ > Sr++ > Mg++ >
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20. Vascular stasis, intestinal hemorrhage, and heightened vascular permeability complicate acute portal hypertension in cd39-null mice
Vasoactive factors that regulate splanchnic hemodynamics include nitric oxide, catecholamines, and possibly extracellular nucleosides/nucleotides (adenosine, ATP). CD39/ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase1) is the major vascular ectonucleotidase that hydrolyzes extracellular nucleotides. CD39 activity may be modulated by vascular injury
American Physiological Society.
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21. Thromboregulatory manifestations in human CD39 transgenic mice and the implications for thrombotic disease and transplantation
Extracellular nucleotides play an important role in thrombosis and inflammation, triggering a range of effects such as platelet activation and recruitment, endothelial cell activation, and vasoconstriction. CD39, the major vascular nucleoside triphosphate diphosphohydrolase (NTPDase), converts ATP and ADP to AMP, which is further degraded to the antithrombot
American Society for Clinical Investigation.
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22. The endothelial cell ecto-ADPase responsible for inhibition of platelet function is CD39.
We previously demonstrated that when platelets are in motion and in proximity to endothelial cells, they become unresponsive to agonists (Marcus, A.J., L.B. Safier, K.A. Hajjar, H.L. Ullman, N. Islam, M.J. Broekman, and A.M. Eiroa. 1991. J. Clin. Invest. 88:1690-1696). This inhibition is due to an ecto-ADPase on the surface of endothelial cells which metabol
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23. Expression of the Virulence Plasmid-Carried Apyrase Gene (apy) of Enteroinvasive Escherichia coli and Shigella flexneri Is under the Control of H-NS and the VirF and VirB Regulatory Cascade
The transcription of the virulence plasmid (pINV)-carried invasion genes of Shigella flexneri and enteroinvasive Escherichia coli (EIEC) is induced at 37°C and repressed at 30°C. In this work, we report that the O135: K−:H− EIEC strain HN280 and S. flexneri SFZM53, M90T, and 454, of serotypes 4, 5, and 2a, respectively, produce apyrase (ATP-diphosphohy
American Society for Microbiology.