Assay Plates
Mostrando 1-12 de 448 artigos, teses e dissertações.
-
1. Rapid and Inexpensive Colorimetric Detection of Total Serum Protein Using Microzone Plates Wax-Printed on Polyester Films
This report describes a rapid and inexpensive colorimetric method based on biuret assay for total serum protein analysis using wax printed microzone plates and a desktop scanner. The colorimetric assay was performed in 96-microzone plates fabricated on polyester films through a wax printing protocol. The image scanning mode and the concentration of the biure
J. Braz. Chem. Soc.. Publicado em: 2021-02
-
2. Ballistic Behavior of New Alumina Composites Reinforced with Niobo-Phosphate Glass
This work aimed to produce and evaluate the ballistic behavior of niobo-phosphate glass-doped alumina composites. The niobo-phosphate glass (30% Nb2O5- 30% P2O5- 20% CaO - 20% CaF2, by mol) were added to the alumina in six different weight percentages. The composite with the highest densification, measured by the Archimedes assay, was used for the ballistic
Mat. Res.. Publicado em: 23/09/2019
-
3. Rapid Detection of Sepsis using CESDA: the Caenorhabditis elegans Sepsis Detection Assay
Abstract INTRODUCTION The nematode Caenorhabditis elegans was used as a biological sensor to detect the urine of sepsis patients (CESDA assay). METHODS C. elegans was aliquoted onto the center of assay plates and allowed to migrate towards sepsis (T) or control (C) urine samples spotted on the same plate. The number of worms found in either (T) or (C) was
Rev. Soc. Bras. Med. Trop.. Publicado em: 14/03/2019
-
4. Effects of mineral trioxide aggregate, BiodentineTM and calcium hydroxide on viability, proliferation, migration and differentiation of stem cells from human exfoliated deciduous teeth
Abstract Objective: The aim of the study was to evaluate the effects of the capping materials mineral trioxide aggregate (MTA), calcium hydroxide (CH) and BiodentineTM (BD) on stem cells from human exfoliated deciduous teeth (SHED) in vitro. Material and Methods: SHED were cultured for 1 – 7 days in medium conditioned by incubation with MTA, BD or CH (1
J. Appl. Oral Sci.. Publicado em: 01/02/2018
-
5. Pectin lyase overproduction by Penicillium griseoroseum mutants resistant to catabolite repression
Abstract Expression of pectinolytic genes is regulated by catabolic repression limiting the production of pectin lyase (PL) if the natural inducer, pectin, is missing from the growth medium. Here, we report the isolation of Penicillium griseoroseum mutants resistant to 2-deoxy-d-glucose (DG) that show resistance to catabolite repression and overproduce PL. T
Braz. J. Microbiol.. Publicado em: 2017-07
-
6. Low-intensity laser phototherapy enhances the proliferation of dental pulp stem cells under nutritional deficiency
Abstract Dental trauma in immature permanent teeth can damage pulp vascularization, which leads to necrosis and cessation of apexogenesis. Studies on tissue engineering using stem cells from human exfoliated deciduous teeth (SHEDs) have yielded promising results. Laser phototherapy (LPT) is able to influence the proliferation and differentiation of these cel
Braz. oral res.. Publicado em: 31/05/2016
-
7. Mixed biofilms formed by C. albicans and non-albicans species: a study of microbial interactions
Abstract Most Candida infections are related to microbial biofilms often formed by the association of different species. The objective of this study was to evaluate the interactions between Candida albicans and non-albicans species in biofilms formed in vitro. The non-albicans species studied were:Candida tropicalis, Candida glabrata andCandida krusei. Singl
Braz. oral res.. Publicado em: 15/03/2016
-
8. Cytotoxic effects of new MTA-based cement formulations on fibroblast-like MDPL-20 cells
Abstract The present study aimed at evaluating the cytotoxic effects of a novel cement called CER on periodontal fibroblast-like cells of mice (MDPL-20), in comparison with different formulations of Mineral Trioxide Aggregate (MTA), by means of the cell viability test (MTT) and cell morphology analysis. Thirty-two round-shaped samples were fabricated with th
Braz. oral res.. Publicado em: 08/03/2016
-
9. Sub-MIC of antibiotics induced biofilm formation of Pseudomonas aeruginosa in the presence of chlorhexidine
Public health is facing a new challenge due to the alarming increase in bacterial resistance to most of the conventional antibacterial agents. It has been found that only minor cell damage is caused when exposed to sub-lethal levels of antimicrobial. Biofilms can play an important role in producing resistance, which is developed to reservoirs of pathogens in
Braz. J. Microbiol.. Publicado em: 2015-03
-
10. Development and validation of a genotype 3 recombinant protein-based immunoassay for hepatitis E virus serology in swine
Hepatitis E virus (HEV) is classified within the family Hepeviridae, genus Hepevirus. HEV genotype 3 (Gt3) infections are endemic in pigs in Western Europe and in North and South America and cause zoonotic infections in humans. Several serological assays to detect HEV antibodies in pigs have been developed, at first mainly based on HEV genotype 1 (Gt1) antig
Braz J Med Biol Res. Publicado em: 21/03/2014
-
11. Influence of substratum position and acquired pellicle on Candida albicans biofilm
The purpose of this study was to evaluate the influence of the substratum position and the saliva acquired pellicle (AP) on Candida albicans biofilm development. Poly(methylmethacrylate) (PMMA) disks were fabricated and randomly allocated to experimental groups: HNP (disks placed in a horizontal position and uncoated by pellicle), VNP (disks placed in a vert
Braz. oral res.. Publicado em: 11/06/2013
-
12. A multiparametric assay to compare the cytotoxicity of different storage media for avulsed teeth
AIM: To evaluate the cytotoxicity of several storage media (coconut water, whole milk, isotonic solution, Hank´s Balanced Salt Solution and tap water) using a multiparametric cytotoxicity analysis employing 3T3 cells. METHODS: Plates containing confluent 3T3 fibroblasts were exposed to the various media for 24h, at 37°C with 5% CO2, and cell viability was
Braz. J. Oral Sci.. Publicado em: 2013-06