Ascospores
Mostrando 13-24 de 127 artigos, teses e dissertações.
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13. Mechanisms of Protection of Trehalase Against Heat Inactivation in Neurospora
The half-life of trehalase and invertase at 65 and 60 C was found to be much greater when intact ascospores of Neurospora tetrasperma were heated, as compared with extracts. By contrast, no protection was afforded these enzymes when they were heated in intact conidia and mycelium of N. crassa or N. tetrasperma. The protective effect of ascospores for trehala
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14. Heat resistance of Byssochlamys ascospores.
Ascospores from 25 strains of Byssochlamys were studied for their ability to resist heat treatment in a standard defined medium. Seven of these were able to survive heating at 90 degrees C for 25 min or longer, when initial numbers were frequently near 10(6)/ml. Ascospores from five resistant strains suspended in the medium at pH 5.0 were usually more resist
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15. Activity and Heat Stability of Trehalase from the Mycelium and Ascospores of Neurospora
Trehalases from the ascospores of Neurospora tetrasperma and the mycelium of N. crassa were compared. Enzymes from both sources have identical electrophoretic mobilities, Km's, responses to pH, immunological reactions, and activities in low-molarity buffers. Because both enzymes are so similar, conclusions about the properties of the ascospore enzyme may, be
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16. Glucose-C14 Metabolism of Dormant and Activated Ascospores of Neurospora
Budd, Kenneth (The University of Michigan, Ann Arbor), Alfred S. Sussman, and Frederick I. Eilers. Glucose-C14 metabolism of dormant and activated ascospores of Neurospora. J. Bacteriol. 91:551–561. 1966.—Dormant and activated ascospores of Neurospora tetrasperma, incubated in C14-labeled glucose, absorb and metabolize this sugar. At the same time, up to
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17. The Mutation SK(ad-3A) Cancels the Dominance of ad-3A+ over ad-3A in the Ascus of Neurospora
A newly induced mutant of Neurospora, when crossed with an ad-3A mutant, produces asci with four viable black and four inviable white ascospores. The survivors always contain the new mutant allele, never ad-3A. The new allele, which is called SK(ad-3A) (for spore killer of ad-3A), is located at or very near the ad-3A locus.—In crosses homozygous for ad-3A,
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18. Some Observations of Ascospores of Neurospora crassa Made with a Scanning Electron Microscope
Scanning electron micrographs of ascospores of Neurospora crassa reveal two of the structures which develop during germination and outgrowth: (i) a germination pore and (ii) the probable site of initiation of hyphal cell wall synthesis.
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19. A New Method To Monitor Airborne Inoculum of the Fungal Plant Pathogens Mycosphaerella brassicicola and Botrytis cinerea
We describe a new microtiter immunospore trapping device (MTIST device) that uses a suction system to directly trap air particulates by impaction in microtiter wells. This device can be used for rapid detection and immunoquantification of ascospores of Mycosphaerella brassicicola and conidia of Botrytis cinerea by an enzyme-linked immunosorbent assay (ELISA)
American Society for Microbiology.
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20. Germination of Saccharomyces cerevisiae ascospores without trehalose mobilization as revealed by in vivo 13C nuclear magnetic resonance spectroscopy.
Saccharomyces cerevisiae ascospores germinate in the presence of acetate without any detectable trehalose degradation, as revealed by high-resolution nuclear magnetic resonance spectroscopy and by a standard colorimetric assay. The results presented here substantiate the hypothesis that in S. cerevisiae trehalose supplies energy during dormancy of the spores
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21. Deletion and Complementation of the Mating Type (MAT) Locus of the Wheat Head Blight Pathogen Gibberella zeae
Gibberella zeae, a self-fertile, haploid filamentous ascomycete, causes serious epidemics of wheat (Triticum aestivum) head blight worldwide and contaminates grain with trichothecene mycotoxins. Anecdotal evidence dating back to the late 19th century indicates that G. zeae ascospores (sexual spores) are a more important inoculum source than are macroconidia
American Society for Microbiology.
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22. Osteomyelitis caused by Neosartorya pseudofischeri.
The first case of osteomyelitis caused by Neosartorya pseudofischeri is reported. The patient, a 77-year-old male with a history of silicosis and tuberculosis, on X-ray examination revealed lytic lesions of L2 and L3 vertebrae suspicious for metastatic lesions. Histologic examination of biopsy specimens from vertebral bodies showed short, distorted, extra- a
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23. Phenotypic Diversity among Alleles at the PER-1 Locus of NEUROSPORA CRASSA
Comparison of 11 perithecial color mutants suggested that all were alleles at the per-1 locus but nonetheless separable into two groups because of phenotypic differences. Three of the mutant strains produced orange perithecia and black ascospores, and eight produced paler, yellow perithecia and white ascospores. Perithecial phenotype was dependent upon the g
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24. Expression of Meiotic Drive Elements Spore Killer-2 and Spore Killer-3 in Asci of Neurospora Tetrasperma
It was shown previously that when a chromosomal Spore killer factor is heterozygous in Neurospora species with eight-spored asci, the four sensitive ascospores in each ascus die and the four survivors are all killers. Sk-2(K) and Sk-3(K) are nonrecombining haplotypes that segregate with the centromere of linkage group III. No killing occurs when either one o