Ascorbate Oxidase
Mostrando 1-12 de 65 artigos, teses e dissertações.
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1. Detection of 1-aminocyclopropane-1-carboxylate oxidase activity in seeds of Stylosanthes humilis H.B.K
The activity of 1-aminocyclopropane-1-carboxylate oxidase (ACO) was characterized in seeds of the tropical legume Townsville stylo (Stylosanthes humilis) both in vitro (desalted extract of non-dormant seeds) and in vivo (entire dormant seeds). Optimum conditions for maximum in vitro ACO activity in a Trizma-HCl 100 mM buffered medium were: pH 7.0, temperatur
Theor. Exp. Plant Physiol.. Publicado em: 2013
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2. Effects of in vitro triacontanol on growth, antioxidant enzymes, and photosynthetic characteristics in Arachis hypogaea L.
In vitro effects of triacontanol (TRIA) on antioxidant enzymes and photosynthetic characteristics were studied in Arachis hypogaea L. cultivars (M-13 and PBS24030). The in vitro impact of TRIA on multiplication potential was also evaluated, which was found to be best at 2.0 ml L-1 TRIA in combination with 6- Benzyl adenine (BA, 3 mg L-1) in both the groundnu
Brazilian Journal of Plant Physiology. Publicado em: 2011
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3. Use of biomimetic systems at the preparation of amperometric sensors / Utilização de sistemas biomiméticos no Preparo de sensores amperométricos
This work describe the immobilization of the copper(II) dinuclear complex ([Cu2(apyhist)2Cl2](ClO4)2), (apyhist =[(4-imidazolyl)ethylene-2-amino-1-ethylpyridine]), and others similar copper(II) compounds mono- and tetranuclears, obtained in water solution or for desprotonation of the imidazole ligant, in the Nafion membrane on glass carbon electrode surface,
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 04/09/2009
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4. Expression analysis of a set of genes related to the ripening of bananas and mangoes
During ripening many important physic-chemical changes contribute to fruit quality, and they are precisely determined by gene expression. Specific genes are essential to normal ripening; however, information on gene expression about the majority of tropical fruit, such as bananas and mangoes is limited. In this way, the present study was undertaken with the
Brazilian Journal of Plant Physiology. Publicado em: 2009
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5. Extração da ascorbato oxidase de Cucurbita maxima por processo descontínuo e contínuo em coluna de discos rotativos perfurados utilizando sistemas de duas fases aquosas / Extraction of ascorbate oxidase from Cucurbita maxima by discontinuous and continuous process in perforated rotating disc contactor using aqueous two-phase systems.
The partition and purification of ascorbate oxidase from pumpkin (Cucurbita maxima) by liquid-liquid extraction in aqueous two-phase system (ATPS) by discontinuous and continuous process, using perforated rotating disc contactor (PRDC) was studied. Experimental designs were used to choose the significant variables for discontinuous process, and polyethylene
Publicado em: 2008
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6. Atividade respiratória e metabolismo antioxidativo em raízes de plântulas de milho (Zea mays L.) submetidas ao estresse salino / Respiration activity and antioxidative metabolism in roots of maize (Zea mays L.) seedlings submitted to salt stress
Tolerance to salt stress was studied in maize seedlings (Zea mays L.) cultivars AGN 3150, BR 106, BR 201, BR 206 and SHS 4040, grown in Hoaglands nutrient solution. Treatments with NaCl 50 and 100 mM led to a decrease in the biomass production and to an increase in the relative electrolyte leakage in the roots and shoots in all cultivars. However, the larges
Publicado em: 2006
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7. Respiration, oxidative phosphorylation, and uncoupling protein in Candida albicans
The respiration, membrane potential (Dy), and oxidative phosphorylation of mitochondria in situ were determined in spheroplasts obtained from Candida albicans control strain ATCC 90028 by lyticase treatment. Mitochondria in situ were able to phosphorylate externally added ADP (200 µM) in the presence of 0.05% BSA. Mitochondria in si
Brazilian Journal of Medical and Biological Research. Publicado em: 2004-10
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8. Coordination environment and fluoride binding of type 2 copper in the blue copper protein ascorbate oxidase
The coordination environment of the type 2 (nonblue) copper in native ascorbate oxidase (L-ascorbate:oxygen oxidoreductase, EC 1.10.3.3) and of a derivative of the enzyme having the type 1 (blue) copper reversibly bleached has been examined by electron paramagnetic resonance (EPR) spectroscopy. In the g[unk] region of the spectrum of bleached ascorbate oxida
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9. Determination of serum cholesterol concentration in the presence of ascorbate.
Pretreatment of a serum or plasma sample with ascorbate oxidase removed interfering ascorbate and allowed the determination of cholesterol to be carried out by a current enzymatic cholesterol method available in kit form. The Cobas-Fara was programmed to carry out pretreatment of the sample with ascorbate oxidase before addition of the cholesterol colour rea
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10. Marked Increase in Ascorbate Oxidase Protein in Pumpkin Callus by Adding Copper 1
Ascorbate oxidase from pumpkin (Cucurbita sp.) was purified from a commercially available preparation. A single polypeptide band with Mr 64,000 was detected after sodium dodecylsulfate-polyacrylamide gel electrophoresis of the purified enzyme. In double immunodiffusion tests, antiserum against the purified preparation formed a single precipitin line with the
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11. Primary structure of cucumber (Cucumis sativus) ascorbate oxidase deduced from cDNA sequence: homology with blue copper proteins and tissue-specific expression.
cDNA clones for ascorbate oxidase were isolated from a cDNA library made from cucumber (Cucumis sativus) fruit mRNA. The library was screened with synthetic oligonucleotides that encode the NH2-terminal sequence of this enzyme. Nucleotide sequence analysis of the cloned cDNA inserts revealed a 1761-base-pair open reading frame that encoded an NH2-terminal si
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12. Terminal branching of the respiratory electron transport chain in Neisseria meningitidis.
The respiratory components of the envelope membrane preparation of Neisseria meningitidis were investigated. Oxidase activities were demonstrated in this fraction in the presence of succinic acid, reduced nicotinamide adenine dinucleotide, and ascorbate-N,N,N',N'-tetramethyl-p-phenylene-diamine (TMPD). Differences in the kinetics of inhibition by terminal ox