Yeast general transcription factor GFI: sequence requirements for binding to DNA and evolutionary conservation.
AUTOR(ES)
Dorsman, J C
RESUMO
GFI is an abundant DNA binding protein in the yeast S. cerevisiae. The protein binds to specific sequences in both ARS elements and the upstream regions of a large number of genes and is likely to play an important role in yeast cell growth. To get insight into the relative strength of the various GFI-DNA binding sites within the yeast genome, we have determined dissociation rates for several GFI-DNA complexes and found them to vary over a 70-fold range. Strong binding sites for GFI are present in the upstream activating sequences of the gene encoding the 40 kDa subunit II of the QH2:cytochrome c reductase, the gene encoding ribosomal protein S33 and in the intron of the actin gene. The binding site in the ARS1-TRP1 region is of intermediate strength. All strong binding sites conform to the sequence 5' RTCRYYYNNNACG-3'. Modification interference experiments and studies with mutant binding sites indicate that critical bases for GFI recognition are within the two elements of the consensus DNA recognition sequence. Proteins with the DNA binding specificities of GFI and GFII can also be detected in the yeast K. lactis, suggesting evolutionary conservation of at least the respective DNA-binding domains in both yeasts.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=330762Documentos Relacionados
- Yeast and human TATA-binding proteins have nearly identical DNA sequence requirements for transcription in vitro.
- The nucleotide sequence of rat alpha-tubulin: 3'-end characteristics, and evolutionary conservation.
- Sequence requirements for terminators and antiterminators in the T box transcription antitermination system: disparity between conservation and functional requirements
- Making (anti)sense of non-coding sequence conservation.
- A new mouse TCR V gamma gene that shows remarkable evolutionary conservation.