Xylitol formation and reduction equivalent generation during anaerobic xylose conversion with glucose as cosubstrate in recombinant Saccharomyces cerevisiae expressing the xyl1 gene.
AUTOR(ES)
Thestrup, H N
RESUMO
Glucose was used as a cosubstrate under anaerobic conditions in the conversion of xylose to xylitol by a recombinant Saccharomyces cerevisiae strain expressing the xyl1 gene. Glucose was metabolized mainly through glycolysis, with carbon dioxide, acetate, and ethanol as end products and with reduction equivalents generated in the glyceraldehyde-3-phosphate dehydrogenase and acetaldehyde dehydrogenase reactions. At a high glucose supply rate, generation of surplus reduction equivalents resulted in simultaneous ethanol formation. On the other hand, at a low glucose supply rate, additional reduction equivalents were generated by simultaneous ethanol consumption. A significantly lower xylitol formation rate was observed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=167474Documentos Relacionados
- Influence of cosubstrate concentration on xylose conversion by recombinant, XYL1-expressing Saccharomyces cerevisiae: a comparison of different sugars and ethanol as cosubstrates.
- A glycerol-3-phosphate dehydrogenase-deficient mutant of Saccharomyces cerevisiae expressing the heterologous XYL1 gene.
- Anaerobic Xylose Fermentation by Recombinant Saccharomyces cerevisiae Carrying XYL1, XYL2, and XKS1 in Mineral Medium Chemostat Cultures
- Deletion of the GRE3 Aldose Reductase Gene and Its Influence on Xylose Metabolism in Recombinant Strains of Saccharomyces cerevisiae Expressing the xylA and XKS1 Genes
- Ethanolic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene, which expresses an active xylose (glucose) isomerase.
