Wnt family proteins are secreted and associated with the cell surface.
AUTOR(ES)
Smolich, B D
RESUMO
Members of the Wnt gene family are proposed to function in both normal development and differentiation as well as in mammary tumorigenesis. To understand the function of Wnt proteins in these two processes, we present here a biochemical characterization of seven Wnt family members. For these studies, AtT-20 cells, a neuroendocrine cell line previously shown to efficiently process and secrete Wnt-1, was transfected with expression vectors encoding Wnt family members. All of the newly characterized Wnt proteins are glycosylated, secreted proteins that are tightly associated with the cell surface or extracellular matrix. We have also identified native Wnt proteins in retinoic acid-treated P19 embryonal carcinoma cells, and they exhibit the same biochemical characteristics as the recombinant proteins. These data suggest that Wnt family members function in cell to cell signaling in a fashion similar to Wnt-1.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=275763Documentos Relacionados
- Secreted int-1 protein is associated with the cell surface.
- Envelope proteins of Semliki Forest virus synthesized in Xenopus oocytes are transported to the cell surface.
- Interaction of papillomaviruses with the cell surface.
- Evidence that the superoxide-generating system of human leukocytes is associated with the cell surface.
- Interaction of outer envelope proteins of Chlamydia psittaci GPIC with the HeLa cell surface.
