Variation of Hydroxyapatite Content in Soft Gelatin Affects Mesenchymal Stem Cell Differentiation

AUTOR(ES)

Kantawong, Fahsai, Tanum, Junijira, Wattanutchariya, Wassanai, Sooksaen, Pat

FONTE

Braz. arch. biol. technol.

DATA DE PUBLICAÇÃO

29/04/2016

RESUMO

Gelatin is a common material used in tissue engineering and hydroxyapatite (HA) has a composition and structure similar to natural bone mineral. HA is also used to increase the adhesion ability of scaffolds. The physical and mechanical properties of gelatin, together with the chemical properties of HA, can affect cell differentiation. The main purpose of this study is to investigate the gene expression of human mesenchymal stem cells (HMSCs) upon culturing on gelatin composite with HA. Low amounts of HA were introduced into the gelatin in order to modulate properties of gelatin. Three types of hydrogel were fabricated by glutaraldehyde crosslinking before lyophilization to produce the porous 3D structure: (1) pure gelatin, (2) 0.5 mg/ml HA in gelatin, and (3) 1 mg/ml HA in gelatin. The fabricated hydrogels were used as scaffolds to cultivate HMSCs for two periods - 24 hours and 3 weeks. The results showed that all types of fabricated hydrogels could be used to cultivate HMSCs. Changes of gene expressions indicated that the HMSCs cultured on the 1 mg/ml HA in gelatin showed neuronal lineage-specific differentiation.

Documentos Relacionados

• Thymbra Spicata Var. Intricata Induces Mesenchymal Stem Cell Proliferation and Osteogenic Differentiation
• HOX gene analysis in the osteogenic differentiation of human mesenchymal stem cells
• bFGF promotes adipocyte differentiation in human mesenchymal stem cells derived from embryonic stem cells
• Induction of in vitro differentiation of rat mesenchymal stem cells overexpressing betacellulin into betapancreatic cells
• Mesenchymal Stem Cell and Nucleus Pulposus Cell Coculture Modulates Cell Profile