Variability among inbred lines and RFLP mapping of sunflower isozymes
AUTOR(ES)
Carrera, Alicia D., Pizarro, G., Poverene, M., Feingold, S., León, A.J., Berry, S.T.
FONTE
Genetics and Molecular Biology
DATA DE PUBLICAÇÃO
2002
RESUMO
Eight isozyme systems were used in this study: acid phosphatase (ACP), alcohol dehydrogenase (ADH), esterase (EST), glutamate dehydrogenase (GDH), malate dehydrogenase (MDH), phosphoglucoisomerase (PGI), 6-phosphogluconate dehydrogenase (PGD), and phosphoglucomutase (PGM). The polymorphism of these enzyme systems was studied in 25 elite inbred lines. A total of 19 loci were identified, but only eight of them were polymorphic in the germplasm tested. The polymorphic index for the eight informative markers ranged from 0.08 to 0.57, with a mean value of 0.36. Five isozyme loci were mapped in F2:3 populations with existing RFLP data. Est-1, Gdh-2 and Pgi-2 were mapped to linkage groups 3, 14 and 9, respectively. As in previous reports, an ACP locus and a PGD locus were found to be linked, both located in linkage group 2 of the public sunflower map.
Documentos Relacionados
- Extracting female inbred lines from commercial sunflower hybrids.
- Relative Variability of Inbred Lines and F1 Hybrids in Lycopersicum Esculentum
- Comparison of RAPD, RFLP, AFLP and SSR markers for diversity studies in tropical maize inbred lines
- Simulation of population size and genome saturation level for genetic mapping of recombinant inbred lines (RILs)
- High Genetic Variability of Herbivore-Induced Volatile Emission within a Broad Range of Maize Inbred Lines1