VariaÃÃo GenÃtica em Aedes aegypti e Aedes albopictus




The invasive mosquitoes Aedes aegypti and Aedes albopictus are world wide important human arboviroses vectors. The Ae. aegypti was introduced in Brazil at 16th century, certainly during the traffic of slavers, while the Ae. albopictus was introduced recently and its distribution is spreading rapidly. These insects show population density variation through the time, because the breeding sites of larvae are irregulars and transitory, which depends on human activities and rain occurrence. The consequence of factors that provide this variation over the genetic structure of Ae. aegypti and Ae. albopictus populations were evaluated in this study. Samples of both species were collected by using ovitraps in two areas from Recife. Nuclear (RAPD) and mitochondrial (RFLP and SSCP of citochrome c oxidase gene) markers were used to analyze the mosquitoes. The results obtained by the two molecular markers were similar and showed a constant and high intrapopulational genetic diversity. On the other hand, the genetic differentiation between populations oscillated in the different samples, with a Fst value ranging from o,146 in Ae. aegytpi at March 2004 to 0,304 at September of the same year. The same pattern of variation was observed in Ae. albopictus. To the mitochondrial gene COII was observed high haplotype diversity in both species. Were identified eleven haplotype in Ae. aegypti and ten haplotype in Ae. albopictus. The range in the haplotype frequencies of the mitochondrial gene in the species reflected demographic factors influencing the genetic structure of Aedes spp. populations


anÃlise de poliformismo genÃtica de populaÃÃes genetica estruturÃÃo genÃtica insetos - aedes aegypti e aedes albopictus

Documentos Relacionados