Validation of a liquid chromatography ultraviolet method for determination of herbicide diuron and its metabolites in soil samples
AUTOR(ES)
FELICIO, ANA LUCIA S.M., MONTEIRO, ALESSANDRA M., ALMEIDA, MARIANA B., MADEIRA, TIAGO B., NIXDORF, SUZANA L., YABE, MARIA JOSEFA S.
FONTE
An. Acad. Bras. Ciênc.
DATA DE PUBLICAÇÃO
25/08/2016
RESUMO
ABSTRACT Diuron is one of the most widely herbicide used worldwide, which can undergo degradation producing three primary metabolites: 3,4-dichlorophenylurea, 3-(3,4-dichlorophenyl)-1-methylurea, and 3,4-dichloroaniline. Since the persistence of diuron and its by-products in ecosystems involves risk of toxicity to environment and human health, a reliable quantitative method for simultaneous monitoring of these compounds is required. Hence, a simple method without preconcentration step was validated for quantitation of diuron and its main metabolites by high performance liquid chromatography with ultraviolet detection. Separation was achieved in less than 11 minutes using a C18 column, mobile phase composed of acetonitrile and water (45:55 v/v) at 0.86 mL min-1 and detection at 254 nm. The validated method using solid-liquid extraction followed by an isocratic chromatographic elution proved to be specific, precise and linear (R2 ˃ 0.99), presenting more than 90% of recovery. The method was successfully applied to quantify diuron and their by-products in soil samples collected in a sugarcane cultivation area, focusing on the environmental control.
Documentos Relacionados
- Validation of a High Performance Liquid Chromatography Method for Aflatoxins Determination in Corn Arepas
- Validation of high performance liquid chromatography method for determination of meloxicam loaded PEGylated nanocapsules
- Validation of a method using solid phase extraction and liquid chromatography for the determination of pesticide residues in groundwaters
- Determination of apalcillin and its metabolites in human body fluids by high-pressure liquid chromatography.
- In Tube Ultrasonic and Air Assisted Liquid-Liquid Microextraction-Gas Chromatography-Mass Spectrometry Determination: a Novel Method for the Determination of Phthalate Esters in Aqueous Samples