Validation of a classification and scoring system for the diagnosis of laryngeal and pharyngeal squamous cell carcinomas by confocal laser endomicroscopy

AUTOR(ES)

Sievert, Matti; Mantsopoulos, Konstantinos; Mueller, Sarina K.; Rupp, Robin; Eckstein, Markus; Stelzle, Florian; Oetter, Nicolai; Maier, Andreas; Aubreville, Marc; Iro, Heinrich; Goncalves, Miguel

FONTE

Brazilian Journal of Otorhinolaryngology

DATA DE PUBLICAÇÃO

2022

RESUMO

Abstract Introduction: Confocal laser endomicroscopy is an optical imaging technique that allows in vivo, real-time, microscope-like images of the upper aerodigestive tract’s mucosa. The assessment of morphological tissue characteristics for the correct differentiation between healthy and malignant suspected mucosa requires strict evaluation criteria. Objective: This study aims to validate an eight-point score for the correct assessment of malignancy. Methods: We performed confocal laser endomicroscopy between March and October 2020 in 13 patients. 197 sequences (11.820 images) originated from the marginal area of pharyngeal and laryngeal carcinomas. Specimens were taken at corresponding locations and analyzed in H&E staining as a standard of reference. A total of six examiners evaluated the sequences based on a scoring system; they were blinded to the histopathological examination. The primary endpoints are sensitivity, specificity, and accuracy. Secondary endpoints are interrater reliability and receiver operator characteristics. Results: Healthy mucosa showed epithelium with uniform size and shape with distinct cytoplasmic membranes and regular vessel architecture. Confocal laser endomicroscopy of malignant cells demonstrated a disorganized arrangement of variable cellular morphology. We calculated an accuracy, sensitivity, specificity, positive predictive value, and negative predictive value of 83.2%, 81.3%, 85.5%, 86.7%, and 79.7%, respectively, with a κ-value of 0.64, and an area under the curve of 0.86. Conclusion: The results confirm that this scoring system is applicable in the laryngeal and pharyngeal mucosa to classify benign and malignant tissue. A scoring system based on defined and reproducible characteristics can help translate this experimental method to broad clinical practice in head and neck diagnosis.

Documentos Relacionados

• Confocal laser endomicroscopy in inflammatory bowel diseases: future, reality or dream?
• Probe-based confocal laser endomicroscopy of the airways: physiological and pathological characteristics of preneoplastic and neoplastic lesions
• Technical Note: First Use of Endonasal Confocal Laser Endomicroscopy – Feasibility and Proof of Concept
• CROSS-CULTURAL ADAPTATION AND VALIDATION OF THE CONSTIPATION SCORING SYSTEM FOR THE BRAZILIAN POPULATION
• Diagnosis of lung cancer by fibreoptic bronchoscopy: problems in the histological classification of non-small cell carcinomas.