Vacinas de administração oral contra diarréia associada à Escherichia coli enteropatogênica baseada em linhagens geneticamente modificadas de Bacillus subtilis / Oral vaccines against diarrhea associated with enteropathogenic Escherichia coli strains based on genetically modified Bacillus subtilis strains

AUTOR(ES)
DATA DE PUBLICAÇÃO

2010

RESUMO

The objective of this work was the construction of genetically modified strains of B. subtilis able to express portions of intimin, the main component involved in colonization by enteropathogenic Escherichia coli strains (EPEC) as a strategy of oral vaccination against infectious diarrhea. The vaccines employed five regions of EPEC intimin and B. subtilis strains expressing recombinant proteins in the cytoplasm. Furthermore, we evaluated the use of spores and vegetative cells as vaccine vehicles for the delivery of recombinant antigens based on an epissomal expression system. The efficacy of the vaccines was demonstrated by: (i) production of systemic (IgG) and mucosal (sIgA) antibody responses to intimin, (ii) neutralizing of intimin expressed by different strains of EPEC by the antibodies generated in immunized animals, and (iii) protection to lethal challenges carried out with EPEC strains using an experimental model based in newborn mice. The results represent an important step in the validation of a new vaccine strategy for the control of enteric pathogens. Moreover, we propose the use of an animal model as a new tool to evaluate the protective potential of vaccines against EPEC.

ASSUNTO(S)

mucosal immunity bacillus subtilis ehec vaccines ehec expression system epec vacinas bacillus subtilis imunidade de mucosas epec sistema de expressão

ACESSO AO ARTIGO

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