Utilization of Taenia crassiceps and Taenia solium metacestodes antigens in ELISA test for the diagnosis of bovine cysticercosis / Antígenos de larvas de Taenia crassiceps e Taenia solium em teste ELISA para diagnóstico da cisticercose bovina

AUTOR(ES)

Lílian Lameck Monteiro

DATA DE PUBLICAÇÃO

2004

RESUMO

This study was carried out with the purpose to develop an serological diagnosis protocol of bovine cysticercosis for the ELISA test using two metacestodes antigens of Taenia crassiceps and three of Taenia solium. There were used 20 sera of experimentally infected cattle with T. saginata eggs, 60 of cattle with natural infection, diagnosed at slaughterhouses, five of cattle negative for cisticercose, reared in isolation, 55 of negative bovine in slaughterhouses and 10 of bovine with actinomicosis (n=2), actinobacilosis (n=1), fasciolosis (n=1) diagnosed at slaughterhouse and of experimentally infected cattle with Anaplasma marginale (n=3), Babesia sp. (n=2) and concomitant infection for Anaplasma marginale and Babesia bovis (n=1). There were used the total and fluid cistic antigens of T. Crassiceps metacestodes and escólex and membrane antigens of T. solium metacestodes in ELISA test, after previous characterization in polyacrylamide gel electrophoresis (PAGE) in gradient 5 to 20%. The better antigen concentration was 1 g and the better sera and conjugated dilutions were 1:25 and 1:5.000, respectively. Although the T. solium antigens have provided the most sensibility values, the T. crassiceps antigens also showed good performance to the bovine cysticercosis diagnosis. Different control serum groups employed for the cut-off calculation had changed the ELISA test results. We can concluded that the ELISA test for antibodies detection presents deficiencies in the diagnosis of naturally infected animals that showed low sensibility (5 to 32%) for different antigens. However, for experimentally infected cattle, the sensibility was high, 75 to 90% for different antigens. The test could still be considered useful in the differentiation between the cisticercose and other diseases, due to its high specificity rates (81 to 100%).

ASSUNTO(S)

bovine cysticercosis taenia saginata teste imunoenzimático cisticercose taenia solium inspecao de produtos de origem animal bovino enzyme-linked immunosorbent assay taenia crassiceps parasito taenia saginata parasite taenia crassiceps taenia solium

Documentos Relacionados

• Teste ELISA para diagnóstico da cisticercose suína usando antígenos de larvas de Taenia solium e Taenia crassiceps
• Antígenos de larva de Taenia solium em ELISA para diagnóstico da cisticercose bovina
• Peptídeos relevantes de Taenia crassiceps para o diagnóstico da cisticercose bovina pelo immunoblot
• Avaliação de antígenos de cisticercos de Taenia solium e Taenia crassiceps para o imunodiagnóstico da neurocisticercose por ELISA em amostras de líquido cefalorraquidiano
• Evaluation of the imunoblotting for the bovine cysticercosis diagnosis