Utilization of Electron Spin Resonance Spectroscopy for Observation of In Vivo Response to Fungicides

AUTOR(ES)

Rowlands, John R.

RESUMO

Electron spin resonance studies of the in vivo response to fungicides (chloranil and thiram) by Aspergillus niger of different ages are described. The spores possessed a natural free radical signal which grew rapidly upon application of chloranil. Thiram, on the other hand, produced weak changes in the free radical region but easily detectable changes at lower fields, indicating involvement of copper and iron. In addition, flow experiments are described in which the production and decay of the chloranil semiquinone radical produced in the reaction of solutions of chloranil with extracts of bakers' yeast were monitored, both in the absence and in the presence of several buffers and respiratory chain inhibitors.

Documentos Relacionados

• Electron-nuclear double resonance spectroscopy (and electron spin-echo envelope modulation spectroscopy) in bioinorganic chemistry
• Myocardial ischemia and reperfusion: direct evidence for free radical generation by electron spin resonance spectroscopy.
• Investigation of Very Slowly Tumbling Spin Labels by Nonlinear Spin Response Techniques: Theory and Experiment for Stationary Electron Electron Double Resonance
• Hydroxyl radical generation by polymorphonuclear leukocytes measured by electron spin resonance spectroscopy.
• In vivo spin-label murine pharmacodynamics using low-frequency electron paramagnetic resonance imaging.