Utilização de regiões genomicas polimorficas para o estudo de similaridade de estafilococos resistentes a oxacilina, com fins epidemiologicos / The use of polimorphic genomic regions for the similarity study of multi resistant Staphylococcus to oxacillin, with epidemiologist aims

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

One hundred and fifty-four methicillin-resistant Staphy/ococcus aureus (MRSA) strains have been isolated from patients attended in tertiary care hospitais in two metropolitan areas (Campinas City and Ribeirão Preto City) in the southeast region of Srazil and analyzed through PCR-based techniques [(PCR amplification of spA, coa, and housekeeping genes (arcC, amE, gmk, pta, tpi, yqiL)] and further restriction fragment typing of coa and housekeeping genes. The heterogeneity of spA gene was determined directly by agarose gel electrophoresis migration. The results obtained indicate the existence of three (A, S, C) main strain clusters. Since the strain distribution in these three clusters is much characteristic, it denotes the existence of three main clones. Ali strains isolated in Campinas were grouped in clusters A and S, while most of the strains isolated in Ribeirão Preto were grouped in cluster C, with a few strains in cluster A (4) and S (1). This distribution denotes the existence of different founder strains that undergo independent genetic variability. The presénce of strains of one city (Ribeirão Preto) with genetic proximity to strains of another city (Campinas) may reflect the possible spread of strains with the acquisition of the infection in one city and the medical treatment in the other one. The strains considered representative of the Srazilian Epidemic Clone (SEC) were categorized as clone A. These results indicate a possible variability higher among Srazilian MRSA strains than currently described

ASSUNTO(S)

oxacilina resistance estafilococos resistencia staphylococcus oxacilln

ACESSO AO ARTIGO

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