Utilização de células mononucleares da medula óssea na regeneração do nervo ciático de ratos após neurorrafia

AUTOR(ES)

João Damasceno Lopes Filho

DATA DE PUBLICAÇÃO

2009

RESUMO

Peripheral nerves injuries are frequent and can result in permanent loss of function. Currently, cell therapy constitutes a great possibility of improving nerve regeneration, increasing the success of the nerve repair. Objective: To evaluate the use of mononuclear cells in the regeneration process of the sciatic nerve after its section and followed by end-to-end neurorrhaphy. Material and Methods: Forty adult male Wistar rats (250 to 300 g) were divided into four groups: 1 - Sham; 2 after nerve section, neurorrhaphy was immediately performed; 3 - after neurorrhaphy, a culture medium was injected into the nerve segment distal to the suture; 4 - after neurorrhaphy, mononuclear cells were injected. Mononuclear cells were obtained from the bone marrow and separated by Ficoll-Hypaque method. The immunohistochemical and histological analyses were perfomed at the 4th postoperative day. The sciatic functional index, histological and morphometric analyses were used to evaluate nerve regeneration at the 6th. Results: The histological analysis showed that the nerves in which mononuclear cells were used presented a more accelerated degenerative process at the 4th postoperative day. According to the immunohistochemical evaluation, an increase of the neurotrophic factors was observed in the same period. In the 6th postoperative week, all the morphometric results of the mononuclear cell group were statistically higher compared to groups 2 and 3. In the 6th postoperative week there was a statistically significant improvement in the sciatic functional index for group 4 (mononuclear cell) compared to groups 2 and 3. Conclusion: Mononuclear cells have stimulated nerve regeneration. These cells have contributed to an increase in the cellularity and the neurotrophic factors in the microenvironment of the axotomized nerve.

ASSUNTO(S)

regeneração nervosa terapia celular cell therapy nervio ciático nervo ciático terapia tecidual bone marrow medula óssea terapia tisular nerve regeneration. médula Ósea tissue therapy regeneración nerviosa ortopedia sciatic nerve

Documentos Relacionados

• O ultrassom terapêutico na medula espinhal acelera a regeneração do nervo ciático de ratos
• Homing miocárdico após infusão intrapericárdica de Células Mononucleares de Medula Óssea
• Utilização de células-tronco autólogas de medula óssea na regeneração do nervo tibial de coelhos mediante técnica de tubulização com prótese de silicone
• Transplante de células mononucleares da medula óssea na epilepsia experimental induzida por lítio e pilocarpina em ratos
• Efeitos do transplante autólogo de células-tronco mononucleares da medula óssea na miocardiopatia induzida pela doxorrubicina em ratos wistar