UtilizaÃÃo de ondas sÃnicas e ultra-sÃnicas para obtenÃÃo de DNA em amostra de mÃsculo humano

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

DNA extraction for forensic issues can be divided into two stages, digestion or lysis of samples, releasing DNA molecules in solution, and purification, making the DNA accessible to other process steps. Methods of cell lysis in current use in forensic laboratories include temperature variations and use of chemicals like proteinase K, detergents and reducing agents in a very slow and high cost process. In this work, two new methods for obtaining DNA from human muscle by using sonic probe (8 kHz) and ultrasonic probes (24 KHz) were developed. By using sonic waves is possible to execute complete lysis of muscle samples in buffer 10 mM Tris-HCl, pH 8.0, SDS 2% in only two minutes. Ultrasonic waves enable DNA extraction in Milli-Q water in two minutes without subsequent purification steps. Both methods resulted in sufficient quantities of DNA for human identification in forensic science, however, sonicated samples resulted DNA quantities significantly larger than samples subjected to ultrasound.

ASSUNTO(S)

extraÃÃo extraction ciencias biologicas dna mÃsculo sonicaÃÃo dna ultra-som ultra-sound sonication muscle

ACESSO AO ARTIGO

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