Uso de genes anti-apoptóticos na construção de plantas de maracujá transgênicas e análise da atividade de diferentes promotores em células vegetais e de insetos

AUTOR(ES)

Daniele Scandiucci de Freitas

DATA DE PUBLICAÇÃO

2006

RESUMO

Plants are usually hosts for more than one pathogen. The passion flower, for example, can be infected by a many virus, bactéria and fungi, which limit fruit yield considerably. Due to this, the use of diseases control systems that allow a wide range of disease resistance is needed. Recent work have shown that tobacco or tomato plants containing anti-apoptotic genes were more resistant to abiotic stresses and several necrotrophic pathogens, which are pathogens that kill the plant host cell as part of their infection process. In this work, the p35 and iap-3 genes from baculoviruses were separetely introduced into the genome of passion fruit plants by biobalistics. The foreign genes were under the transcriptional control of the 35S or UBQ3 promoters. The transgenic plants were regenerated and screened for the presence of the transgene by PCR and/or Southern blot. Only p35+ plants were confirmed by PCR and/or Sothern-blot analysis. Eleven regenerated plants showed the presence of the p35 gene. Transcriptional analysis of regenerated plants showed the presence of specific p35 transcripts in 9 of them. Regenerated plants containing the p35 gene were inoculated with the cowpea aphid-borne mosaic virus (CABMV), the bacterium Xanthomonas axonopodis pv passiflorae, and the herbicide, glufosinate, (Syngenta). None of the plants showed resistance to CABMV. Regenerated plants (p35+) showed less than half of local lesions showed by non-transgenic plants when inoculated with X. axonopodis and some p35+ plants showed increased tolerance to the glufosinate herbicide when compared to non-transgenic plants. Furthermore, we have shown that a baculovirus promoter (IE-1 promoter) is active in plant cells and promoters used in plant biotechnology (UBQ and 35S) are also active in insect cells by transient expression assays using the luciferase gene (rluc) from Renilla reniformis under the transcriptional control of each promoter.

ASSUNTO(S)

biologia molecular baculovirus plantas transgênicas células p35 maracujá ie-1

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