Use of second-site suppressor mutations in Drosophila to identify components of the transcriptional machinery.

AUTOR(ES)

Mortin, M A

RESUMO

Isolation of second-site suppressor mutations provides a powerful method for identifying (i) genes that encode proteins that interact and (ii) domains within the interacting proteins that contact each other. Flies conditionally lethal because they carry mutations in the largest subunit of RNA polymerase II were mutagenized; ten million progeny were then screened for compensatory mutations. Eight intragenic and 10 extragenic suppressor mutations were recovered. Both the conditional lethality and premature termination of transcription caused by one mutation in the largest subunit of RNA polymerase II are compensated by an allele-specific suppressor mutation in the second-largest subunit of the enzyme.

Documentos Relacionados

• Mechanism of rescue of common p53 cancer mutations by second-site suppressor mutations
• The Triplo-Lethal Locus of Drosophila: Reexamination of Mutants and Discovery of a Second-Site Suppressor
• Second-Site Suppressor Mutations of Inactivating Substitutions at Gly247 of the Tetracycline Efflux Protein, Tet(B)
• Second-site suppressor mutations assist in studying the function of the 3' noncoding region of turnip yellow mosaic virus RNA.
• Putative Interhelical Interactions within the PheP Protein Revealed by Second-Site Suppressor Analysis