Use of Multiplex PCR and PCR Restriction Enzyme Analysis for Detection and Exploration of the Variability in the Free-Living Amoeba Naegleria in the Environment

AUTOR(ES)

Pélandakis, Michel

FONTE

American Society for Microbiology

RESUMO

A multiplex PCR was developed to simultaneously detect Naegleria fowleri and other Naegleria species in the environment. Multiplex PCR was also capable of identifying N. fowleri isolates with internal transcribed spacers of different sizes. In addition, restriction fragment length polymorphism analysis of the PCR product distinguished the main thermophilic Naegleria species from the sampling sites.

Documentos Relacionados

• Genetic Variation in the Free-Living Amoeba Naegleria fowleri
• PCR Detection and Analysis of the Free-Living Amoeba Naegleria in Hot Springs in Yellowstone and Grand Teton National Parks
• Elastase in the pathogenic free-living amoebae Naegleria and Acanthamoeba spp.
• The use of singleplex and nested PCR to detect Batrachochytrium dendrobatidis in free-living frogs
• Comparative antigenic analysis of pathogenic and free-living Naegleria species by the gel diffusion and immunoelectrophoresis techniques.