Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA

AUTOR(ES)

Santos, Carla R., Franciscatto, Laura G., Barcellos, Regina B., Almeida, Sabrina E. M, Rossetti, Maria Lucia R.

FONTE

Brazilian Journal of Microbiology

DATA DE PUBLICAÇÃO

2012-03

RESUMO

This study aimed to evaluate the use of the FTA elute cardTM impregnated with cervicovaginal sample directly in the PCR amplification for detection of HPV-DNA. The results were compared to a reference technique. This method was more efficient than the protocol indicated by the manufacturer, identifying 91.7% against 54.2% of the positive samples.

Documentos Relacionados

• Detection of human papillomavirus DNA in prostate gland tissue by using the polymerase chain reaction amplification assay.
• Comparison of Southern blot hybridization and polymerase chain reaction methods for the detection of human papillomavirus DNA.
• Detection by polymerase chain reaction amplification of human herpesvirus 6 DNA in peripheral blood of patients with exanthem subitum.
• Use of multiple displacement amplification in the investigation of human papillomavirus physical status
• Sensitive detection of DNA polymorphisms by the serial invasive signal amplification reaction