Use of ethanol for selective isolation of sporeforming microorganisms.

AUTOR(ES)

Koransky, J R

RESUMO

When mixed cultures containing sporeforming bacteria were treated with heat or with ethanol, the latter consistently resulted in better recovery of Clostridium and Bacillus species. Both techniques were effective in eliminating vegetative cells. An ethanol concentration greater than 25% and exposure for 45 min or longer were necessary to kill all vegetative cells in mixed-culture samples. Ethanol treatment (50% ethanol for 1 h) was effective for isolating sporeforming bacteria from intestinal specimens. Seven different species of Clostridium were the only bacteria isolated from the ethanol-treated specimen of intestinal contents from the large bowel of a patient. It was concluded that treatment with ethanol for 1 h is an effective technique for selective isolation of sporeforming bacteria from mixed cultures and certain types of clinical specimens.

Documentos Relacionados

• Improved chamber for the isolation of anaerobic microorganisms.
• Use of solid-phase PCR for enhanced detection of airborne microorganisms.
• Automated systems for identification of microorganisms.
• Use of a computer to group actinomycetes for studies on the ecology of soil microorganisms.
• Chemically defined medium for oral microorganisms.