Use of a restriction enzyme-digested PCR product as substrate for helicase assays
AUTOR(ES)
Shin, Jae-Ho
FONTE
Oxford University Press
RESUMO
DNA helicases play essential roles in many cellular processes. The currently available techniques to generate substrates for helicase assays are fairly complicated and need some expertise not available in all laboratories. Here, a PCR-based method to generate a substrate for a helicase assay is described, and its application for several archaeal, bacterial and viral enzymes is demonstrated.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=546180Documentos Relacionados
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