Urea-induced structural changes in chromatin obtained by sedimentation.

AUTOR(ES)

Harrington, R E

RESUMO

Sedimentation coefficients have been determined for fractionated preparations of whole and stripped (depleted of very lysine-rich histones and non-histone proteins) chicken erythrocyte chromatin fragments in 0-10 M urea. Significant differences in urea effects are observed between these preparations; differences which can be interpreted structurally by use of Kirkwood's dynamical theory of the translational frictional coefficient. This type of analysis implies that urea-induced chain-swelling in stripped chromatin is due largely to the urea effect upon the constituent nu-bodies, whereas the much larger swelling observed in whole chromatin appears to involve also the effect of urea upon the region between adjacent nu-bodies.

Documentos Relacionados

• Urea-induced release of heat-labile enterotoxin from Escherichia coli.
• Urea-induced binding of histone 1 to nucleosomes lacking linker DNA.
• Counteraction of urea-induced protein denaturation by trimethylamine N-oxide: A chemical chaperone at atomic resolution
• The role of urea-induced osmotic diuresis and hypernatremia in a critically ill patient: case report and literature review
• Growth inhibition of Ureaplasma urealyticum by the proton pump inhibitor lansoprazole: direct attribution to inhibition by lansoprazole of urease activity and urea-induced ATP synthesis in U. urealyticum.